Soybean FGAM synthase promoters direct ectopic nematode feeding site activity

Zarir E. Vaghchhipawala, Jessica A. Schlueter, Randy C. Shoemaker, Sally A. Mackenzie

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Soybean cyst nematode (SCN) resistance in soybean is a complex oligogenic trait. One of the most important nematode resistance genes, rhg1, has been mapped to a distal region of molecular linkage group G in soybean. A simplified genetic system to identify soybean genes with modified expression in response to SCN led to the identification of several genes within the nematode feeding sites. The genes were mapped to reveal their linkage relationship to known QTLs associated with soybean cyst nematode (SCN) resistance. One candidate, a phosphoribosylformylglycinamidine (FGAM) synthase (EC 6.3.5.3) gene, mapped to the same genomic interval as the major SCN resistance gene rhg1 within linkage group G. Isolation of FGAM synthase from a soybean bacterial artificial chromosome (BAC) library revealed two highly homologous paralogs. The genes appeared to be well conserved between bacteria and humans. Promoter analysis of the two soybean homologs was carried out with the Arabidopsis thaliana - Heterodera schachtii system to investigate gene response to nematode feeding. The two promoters and their derived deletion constructions effected green fluorescent protein (GFP) expression within nematode feeding sites. The 1.0-kb promoter sequence immediately adjacent to the translation start site was sufficient to direct expression of GFP within syncytia. A wound-inducible element and a floral organ expression sequence were also identified within these promoters. Although a nematode-responsive element could not be identified, the observed expression of GFP within feeding sites supports the hypothesis that plant gene expression is redirected within feeding sites to benefit the parasite.

Original languageEnglish (US)
Pages (from-to)404-413
Number of pages10
JournalGenome
Volume47
Issue number2
DOIs
StatePublished - Apr 2004

Keywords

  • FGAM synthase
  • Heterodera schachtii
  • Promoter analysis
  • Soybean cyst nematode
  • Syncytium

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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    Vaghchhipawala, Z. E., Schlueter, J. A., Shoemaker, R. C., & Mackenzie, S. A. (2004). Soybean FGAM synthase promoters direct ectopic nematode feeding site activity. Genome, 47(2), 404-413. https://doi.org/10.1139/g03-110