Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic mice

Kay Uwe Wagner, Toni Ward, Barbara Davis, Roger Wiseman, Lothar Hennighausen

Research output: Contribution to journalArticle

223 Scopus citations

Abstract

Cre-1oxP based gene deletion approaches hold great promise to enhance our understanding of molecular pathways controlling mammary development and breast cancer. We reported earlier the generation of transgenic mice that express the Cre recombinase under the control of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR). These mice have become a valuable research tool to delete genes specifically in the mammary gland, other secretory organs, and the female germline. We have now characterized in depth the expression of the MMTV-Cre transgene using the ROSA26-1ox-Stop-1ox-LacZ reporter strain to determine the temporal and spatial activation of Cre on the level of single cells. Our results show that MMTV-mediated Cre-activation is restricted to specific cell types of various secretory tissues and the hematopoietic system. Secondly, the timing of Cre expression varies between tissues and cell types. Some tissues express Cre during embryonic development, while other selected cell types highly activate Cre around puberty, suggesting a strong influence of steroid hormones on the transcriptional activation of the MMTV-LTR. Thirdly, Cre expression in the female germline is restricted to individual mouse lines and is therefore dependent on the site of integration of the transgene. Information provided by this study will guide the researcher to those cell types and developmental stages at which a phenotype can be expected upon deletion of relevant genes.

Original languageEnglish (US)
Pages (from-to)545-553
Number of pages9
JournalTransgenic Research
Volume10
Issue number6
DOIs
StatePublished - Dec 1 2001

Keywords

  • Cre
  • MMTV-LTR
  • Mammary
  • ROSA26
  • Transgenic mice

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

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