Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization

Yilun Cheng; Rachel K. Burrack; Qingsheng Li

doi:10.1369/00221554221114174

Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization

Yilun Cheng, Rachel K. Burrack, Qingsheng Li

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Highly multiplexed protein and RNA in situ detection on a single tissue section concurrently is highly desirable for both basic and applied biomedical research. CO-detection by inDEXing (CODEX) is a new and powerful platform to visualize up to 60 protein biomarkers in situ, and RNAscope in situ hybridization (RNAscope) is a novel RNA detection system with high sensitivity and unprecedent specificity at a single-cell level. Nevertheless, to our knowledge, the combination of CODEX and RNAscope remained unreported until this study. Here, we report a simple and reproducible combination of CODEX and RNAscope. We also determined the cross-reactivities of CODEX anti-human antibodies to rhesus macaques, a widely used animal model of human disease.

Original language	English (US)
Pages (from-to)	571-581
Number of pages	11
Journal	Journal of Histochemistry and Cytochemistry
Volume	70
Issue number	8
DOIs	https://doi.org/10.1369/00221554221114174
State	Published - Aug 2022
Externally published	Yes

Keywords

CODEX
RNAscope ISH
spatial information

ASJC Scopus subject areas

Anatomy
Histology

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10.1369/00221554221114174

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title = "Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization",

abstract = "Highly multiplexed protein and RNA in situ detection on a single tissue section concurrently is highly desirable for both basic and applied biomedical research. CO-detection by inDEXing (CODEX) is a new and powerful platform to visualize up to 60 protein biomarkers in situ, and RNAscope in situ hybridization (RNAscope) is a novel RNA detection system with high sensitivity and unprecedent specificity at a single-cell level. Nevertheless, to our knowledge, the combination of CODEX and RNAscope remained unreported until this study. Here, we report a simple and reproducible combination of CODEX and RNAscope. We also determined the cross-reactivities of CODEX anti-human antibodies to rhesus macaques, a widely used animal model of human disease.",

keywords = "CODEX, RNAscope ISH, spatial information",

author = "Yilun Cheng and Burrack, {Rachel K.} and Qingsheng Li",

note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported in part by NIH R01 AI136756 (Y.L., Q.L.), R01 DK087625-01 (L.Q.), R21 AI143405 (Q.L.). Publisher Copyright: {\textcopyright} The Author(s) 2022.",

year = "2022",

month = aug,

doi = "10.1369/00221554221114174",

language = "English (US)",

volume = "70",

pages = "571--581",

journal = "Journal of Histochemistry and Cytochemistry",

issn = "0022-1554",

publisher = "Histochemical Society Inc.",

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AU - Cheng, Yilun

AU - Burrack, Rachel K.

AU - Li, Qingsheng

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported in part by NIH R01 AI136756 (Y.L., Q.L.), R01 DK087625-01 (L.Q.), R21 AI143405 (Q.L.). Publisher Copyright: © The Author(s) 2022.

PY - 2022/8

Y1 - 2022/8

N2 - Highly multiplexed protein and RNA in situ detection on a single tissue section concurrently is highly desirable for both basic and applied biomedical research. CO-detection by inDEXing (CODEX) is a new and powerful platform to visualize up to 60 protein biomarkers in situ, and RNAscope in situ hybridization (RNAscope) is a novel RNA detection system with high sensitivity and unprecedent specificity at a single-cell level. Nevertheless, to our knowledge, the combination of CODEX and RNAscope remained unreported until this study. Here, we report a simple and reproducible combination of CODEX and RNAscope. We also determined the cross-reactivities of CODEX anti-human antibodies to rhesus macaques, a widely used animal model of human disease.

AB - Highly multiplexed protein and RNA in situ detection on a single tissue section concurrently is highly desirable for both basic and applied biomedical research. CO-detection by inDEXing (CODEX) is a new and powerful platform to visualize up to 60 protein biomarkers in situ, and RNAscope in situ hybridization (RNAscope) is a novel RNA detection system with high sensitivity and unprecedent specificity at a single-cell level. Nevertheless, to our knowledge, the combination of CODEX and RNAscope remained unreported until this study. Here, we report a simple and reproducible combination of CODEX and RNAscope. We also determined the cross-reactivities of CODEX anti-human antibodies to rhesus macaques, a widely used animal model of human disease.

KW - CODEX

KW - RNAscope ISH

KW - spatial information

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ER -

Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization

Abstract

Keywords

ASJC Scopus subject areas

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