Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization

Yilun Cheng, Rachel K. Burrack, Qingsheng Li

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Highly multiplexed protein and RNA in situ detection on a single tissue section concurrently is highly desirable for both basic and applied biomedical research. CO-detection by inDEXing (CODEX) is a new and powerful platform to visualize up to 60 protein biomarkers in situ, and RNAscope in situ hybridization (RNAscope) is a novel RNA detection system with high sensitivity and unprecedent specificity at a single-cell level. Nevertheless, to our knowledge, the combination of CODEX and RNAscope remained unreported until this study. Here, we report a simple and reproducible combination of CODEX and RNAscope. We also determined the cross-reactivities of CODEX anti-human antibodies to rhesus macaques, a widely used animal model of human disease.

Original languageEnglish (US)
Pages (from-to)571-581
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume70
Issue number8
DOIs
StatePublished - Aug 2022

Keywords

  • CODEX
  • RNAscope ISH
  • spatial information

ASJC Scopus subject areas

  • Anatomy
  • Histology

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