@article{d14e52bf5cba40ca9bad8d8c91b52d2f,
title = "Spatiotemporal Characterizations of Spontaneously Beating Cardiomyocytes with Adaptive Reference Digital Image Correlation",
abstract = "We developed an Adaptive Reference-Digital Image Correlation (AR-DIC) method that enables unbiased and accurate mechanics measurements of moving biological tissue samples. We applied the AR-DIC analysis to a spontaneously beating cardiomyocyte (CM) tissue, and could provide correct quantifications of tissue displacement and strain for the beating CMs utilizing physiologically-relevant, sarcomere displacement length-based contraction criteria. The data were further synthesized into novel spatiotemporal parameters of CM contraction to account for the CM beating homogeneity, synchronicity, and propagation as holistic measures of functional myocardial tissue development. Our AR-DIC analyses may thus provide advanced non-invasive characterization tools for assessing the development of spontaneously contracting CMs, suggesting an applicability in myocardial regenerative medicine.",
author = "Akankshya Shradhanjali and Riehl, {Brandon D.} and Bin Duan and Ruiguo Yang and Lim, {Jung Yul}",
note = "Funding Information: We thank the funding support given to J.Y.L. from NSF CAREER award (1351570, PI: Lim), NE DHHS Stem Cell Research Project (2018-07, PI: Lim), UNL Layman New Directions Award (PI: Lim), NIH/NIGMS Nebraska Center for the Prevention of Obesity Diseases (NPOD) Seed Grant (P20GM104320, PI: Zempleni), and NIH/ NIGMS Great Plains IDeA-CTR Pilot Grant (1U54GM115458-01, PI: Rizzo); funding support given to B.D. from AHA Scientist Development Grant (17SDG33680170, PI: Duan); funding support given to R.Y. from NIH/ NIGMS Nebraska Center for Integrated Biomolecular Communication (NCIBC) (P20GM113126, PI: Takacs), NIH/NIGMS Nebraska Center for Nanomedicine (P30GM127200, PI: Bronich), NSF grant (1826135, PI: Yang), and the Nebraska Collaborative Initiative (PI: Yang). Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-54768-w",
language = "English (US)",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}