TY - JOUR
T1 - Species and Trichothecene Genotype of Pathogens Causing Fusarium Head Blight of Wheat in Nebraska, U.S.A.
AU - Valverde-Bogantes, Esteban
AU - Bianchini, Andreia
AU - Wegulo, Stephen N.
AU - Hallen-Adams, Heather E.
N1 - Funding Information:
Funding: This research was funded in part by the Nebraska Wheat Board and the Nebraska Agricultural Experiment Station Hatch Multistate Research capacity funding program from the USDA National Institute of Food and Agriculture (accession no. 1006715).
Publisher Copyright:
© 2021 The American Phytopathological Society
PY - 2021
Y1 - 2021
N2 - Fusarium head blight (FHB) is an economically important disease caused by several Fusarium species affecting wheat and other small grain cereals. In recent years, reports of shifts in populations of FHB pathogens around the world have shown that these populations are dynamic and change continuously, often resulting in increased yield losses or changes in the mycotoxins pro duced in the grain, which highlights the need for increased vigilance. The objective of this research was to identify the species and trichothecene genotypes of FHB pathogens in Nebraska in order to monitor their populations and the major toxigenic risks in the state. A total of 74 single-spore Fusarium isolates were obtained from 42 FHB-symptomatic wheat spikes collected from Nebraska fields during the growing seasons in 2015 to 2018. Most of the isolates were identified as F. graminearum (n 5 67) based on translation elongation factor 1a (TEF1),trichothecene 3-O-acetyltransferase (TRI101), and reductase (RED) sequences. Additional species included F. boothii (n 5 3), F. poae (n 5 2), F. acuminatum (n 5 1), and one isolate was an F. graminearum × F. boothii interspecific hybrid. All F. graminearum and F. boothii isolates had the 15-ADON trichothecene genotype. This study shows that F. graminearum is not the only pathogen causing FHB in Nebraska and helps expand knowledge on the worldwide distribution of F. boothii.
AB - Fusarium head blight (FHB) is an economically important disease caused by several Fusarium species affecting wheat and other small grain cereals. In recent years, reports of shifts in populations of FHB pathogens around the world have shown that these populations are dynamic and change continuously, often resulting in increased yield losses or changes in the mycotoxins pro duced in the grain, which highlights the need for increased vigilance. The objective of this research was to identify the species and trichothecene genotypes of FHB pathogens in Nebraska in order to monitor their populations and the major toxigenic risks in the state. A total of 74 single-spore Fusarium isolates were obtained from 42 FHB-symptomatic wheat spikes collected from Nebraska fields during the growing seasons in 2015 to 2018. Most of the isolates were identified as F. graminearum (n 5 67) based on translation elongation factor 1a (TEF1),trichothecene 3-O-acetyltransferase (TRI101), and reductase (RED) sequences. Additional species included F. boothii (n 5 3), F. poae (n 5 2), F. acuminatum (n 5 1), and one isolate was an F. graminearum × F. boothii interspecific hybrid. All F. graminearum and F. boothii isolates had the 15-ADON trichothecene genotype. This study shows that F. graminearum is not the only pathogen causing FHB in Nebraska and helps expand knowledge on the worldwide distribution of F. boothii.
KW - 15-adon
KW - Fusarium boothii
KW - Fusarium graminearum
KW - Plant disease
KW - Wheat
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U2 - 10.1094/PHP-02-21-0020-RS
DO - 10.1094/PHP-02-21-0020-RS
M3 - Article
AN - SCOPUS:85123797696
SN - 1535-1025
VL - 22
SP - 509
EP - 515
JO - Plant Health Progress
JF - Plant Health Progress
IS - 4
ER -