Specific DNA Amplification Utilizing the Polymerase Chain Reaction and Random Oligonucleotide Primers: Application to the Analysis of Antigen Receptor Variable Regions

William V. Williams, Alice Sato, Milton Rossman, Qiong Fang, David B. Weiner

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The polymerase chain reaction (PCR) allows rapid amplification of DNA of known sequence. In many situations, part of a genetic sequence is known, but adjacent sequences of interest are unknown. This is common in investigations of antigen receptor genes from B and T lymphocytes, which are composed of a constant region of known sequence and a variable region, for which the sequence may not be known. Herein is described a method to amplify DNA when sequence information is available for only one primer. This procedure utilizes a primer of known sequence in conjunction with a mixture of short random primers. Application of this method to the amplification of T-cell antigen receptor cDNA is described.

Original languageEnglish (US)
Pages (from-to)707-720
Number of pages14
JournalDNA and Cell Biology
Volume11
Issue number9
DOIs
StatePublished - Nov 1992
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Specific DNA Amplification Utilizing the Polymerase Chain Reaction and Random Oligonucleotide Primers: Application to the Analysis of Antigen Receptor Variable Regions'. Together they form a unique fingerprint.

Cite this