Specific fragmentation of T7 phage DNA at low-melting sites

V. M. Pavlov, Yu L.L. Lyubchenko, A. S. Borovik, Yu S. Lazurkin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.

Original languageEnglish (US)
Pages (from-to)4053-4062
Number of pages10
JournalNucleic acids research
Volume4
Issue number11
DOIs
StatePublished - Nov 1977

ASJC Scopus subject areas

  • Genetics

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