Specific fragmentation of T7 phage DNA at low-melting sites

V. M. Pavlov; Yu L.L. Lyubchenko; A. S. Borovik; Yu S. Lazurkin

doi:10.1093/nar/4.11.4053

Specific fragmentation of T7 phage DNA at low-melting sites

V. M. Pavlov, Yu L.L. Lyubchenko, A. S. Borovik, Yu S. Lazurkin

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.

Original language	English (US)
Pages (from-to)	4053-4062
Number of pages	10
Journal	Nucleic acids research
Volume	4
Issue number	11
DOIs	https://doi.org/10.1093/nar/4.11.4053
State	Published - Nov 1977

ASJC Scopus subject areas

Genetics

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title = "Specific fragmentation of T7 phage DNA at low-melting sites",

abstract = "A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.",

author = "Pavlov, {V. M.} and Lyubchenko, {Yu L.L.} and Borovik, {A. S.} and Lazurkin, {Yu S.}",

year = "1977",

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AU - Pavlov, V. M.

AU - Lyubchenko, Yu L.L.

AU - Borovik, A. S.

AU - Lazurkin, Yu S.

PY - 1977/11

Y1 - 1977/11

N2 - A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.

AB - A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.

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