TY - JOUR
T1 - Specifically alkylated DNA fragments. Synthesis and physical characterization of D[cGC(o2Me)GCG]and D[cGT(o6Me) GCG]
AU - Kuzmich, Sandra
AU - Marky, Luis A.
AU - Jones, Roger A.
N1 - Funding Information:
ACKNOWLEDGMENTS The authors thank S. Hadden and B.L. Gaffney for excellent technical assistance and Dr. Kenneth J. Breslauer for use of his laboratory facilities. This work was supported by grants from the Rutgers Research Council, the Biomedical Research Support Grant, the American Cancer Society (#CH-248), and the National Institutes of Health (Grant GM 23509, KJB).
PY - 1983/5/25
Y1 - 1983/5/25
N2 - Two hexamer DNA fragments containing a carcinogenic modified base, 06-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were 0o protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40o lower than the unmodified parent compound, d(CG)3. Moreover, the presence of the (06Me)G appears to inhibit the B+Z transition, as determined by CD spectroscopy
AB - Two hexamer DNA fragments containing a carcinogenic modified base, 06-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were 0o protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40o lower than the unmodified parent compound, d(CG)3. Moreover, the presence of the (06Me)G appears to inhibit the B+Z transition, as determined by CD spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=0021112051&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021112051&partnerID=8YFLogxK
U2 - 10.1093/nar/11.10.3393
DO - 10.1093/nar/11.10.3393
M3 - Article
C2 - 6856460
AN - SCOPUS:0021112051
VL - 11
SP - 3393
EP - 3404
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 10
ER -