Specifically alkylated DNA fragments. Synthesis and physical characterization of D[cGC(o2Me)GCG]and D[cGT(o6Me) GCG]

Sandra Kuzmich, Luis A. Marky, Roger A. Jones

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Two hexamer DNA fragments containing a carcinogenic modified base, 06-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were 0o protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40o lower than the unmodified parent compound, d(CG)3. Moreover, the presence of the (06Me)G appears to inhibit the B+Z transition, as determined by CD spectroscopy

Original languageEnglish (US)
Pages (from-to)3393-3404
Number of pages12
JournalNucleic acids research
Volume11
Issue number10
DOIs
StatePublished - May 25 1983

ASJC Scopus subject areas

  • Genetics

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