Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae

Margaret I. Boulton; Wallace G. Buchholz; Melanie S. Marks; Peter G. Markham; Jeffrey W. Davies

doi:10.1007/BF00017445

Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae

Margaret I. Boulton, Wallace G. Buchholz, Melanie S. Marks, Peter G. Markham, Jeffrey W. Davies

Biological Process Development Facility

Research output: Contribution to journal › Article

70 Scopus citations

Abstract

Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.

Original language	English (US)
Pages (from-to)	31-40
Number of pages	10
Journal	Plant Molecular Biology
Volume	12
Issue number	1
DOIs	https://doi.org/10.1007/BF00017445
State	Published - Jan 1989

Keywords

Agrobacterium
Gramineae
agroinfection
maize
maize streak virus
strains

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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Boulton, M. I., Buchholz, W. G., Marks, M. S., Markham, P. G., & Davies, J. W. (1989). Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. Plant Molecular Biology, 12(1), 31-40. https://doi.org/10.1007/BF00017445

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title = "Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae",

abstract = "Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.",

keywords = "Agrobacterium, Gramineae, agroinfection, maize, maize streak virus, strains",

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T1 - Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae

AU - Boulton, Margaret I.

AU - Buchholz, Wallace G.

AU - Marks, Melanie S.

AU - Markham, Peter G.

AU - Davies, Jeffrey W.

PY - 1989/1

Y1 - 1989/1

N2 - Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.

AB - Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.

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