Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae

Margaret I. Boulton, Wallace G. Buchholz, Melanie S. Marks, Peter G. Markham, Jeffrey W. Davies

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalPlant Molecular Biology
Volume12
Issue number1
DOIs
StatePublished - Jan 1989

Keywords

  • Agrobacterium
  • Gramineae
  • agroinfection
  • maize
  • maize streak virus
  • strains

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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