Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy

Raquel O. Rocha, Richard A. Wilson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Electron microscopy (EM) allows characterization of the morphology and ultrastructure of a cell. However, challenges concerning cryo sample fixation are still one of the main roadblocks to its widespread adoption. In this protocol, we describe two alternative EM preparation methods employed to study Magnaporthe oryzae appressoria on artificial hydrophobic surfaces.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages79-85
Number of pages7
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2356
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Appressorium
  • Electron microscopy
  • Imaging
  • Magnaporthe oryzae
  • Sample preparation
  • SEM
  • Surfaces
  • TEM

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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