Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory

Yong Le Pan; Steven C. Hill; Joshua L. Santarpia; Kelly Brinkley; Todd Sickler; Mark Coleman; Chatt Williamson; Kris Gurton; Melvin Felton; Ronald G. Pinnick; Neal Baker; Jonathan Eshbaugh; Jerry Hahn; Emily Smith; Ben Alvarez; Amber Prugh; Warren Gardner

doi:10.1364/OE.22.008165

Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory

Yong Le Pan, Steven C. Hill, Joshua L. Santarpia, Kelly Brinkley, Todd Sickler, Mark Coleman, Chatt Williamson, Kris Gurton, Melvin Felton, Ronald G. Pinnick, Neal Baker, Jonathan Eshbaugh, Jerry Hahn, Emily Smith, Ben Alvarez, Amber Prugh, Warren Gardner

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A system for measuring spectrally-resolved fluorescence cross sections of single bioaerosol particles has been developed and employed in a biological safety level 3 (BSL-3) facility at Edgewood Chemical and Biological Center (ECBC). It is used to aerosolize the slurry or solution of live agents and surrogates into dried micron-size particles, and to measure the fluorescence spectra and sizes of the particles one at a time. Spectrallyresolved fluorescence cross sections were measured for (1) bacterial spores: Bacillus anthracis Ames (BaA), B. atrophaeus var. globigii (BG) (formerly known as Bacillus globigii), B. thuringiensis israelensis (Bti), B. thuringiensis kurstaki (Btk), B. anthracis Sterne (BaS); (2) vegetative bacteria: Escherichia coli (E. coli), Pantoea agglomerans (Eh) (formerly known as Erwinia herbicola), Yersinia rohdei (Yr), Yersinia pestis CO92 (Yp); and (3) virus preparations: Venezuelan equine encephalitis TC83 (VEE) and the bacteriophage MS2. The excitation wavelengths were 266 nm, 273 nm, 280 nm, 365 nm and 405 nm.

Original language	English (US)
Pages (from-to)	8165-8189
Number of pages	25
Journal	Optics Express
Volume	22
Issue number	7
DOIs	https://doi.org/10.1364/OE.22.008165
State	Published - 2014

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OE.22.008165

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Pan, Y. L., Hill, S. C., Santarpia, J. L., Brinkley, K., Sickler, T., Coleman, M., Williamson, C., Gurton, K., Felton, M., Pinnick, R. G., Baker, N., Eshbaugh, J., Hahn, J., Smith, E., Alvarez, B., Prugh, A., & Gardner, W. (2014). Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory. Optics Express, 22(7), 8165-8189. https://doi.org/10.1364/OE.22.008165

Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory. / Pan, Yong Le; Hill, Steven C.; Santarpia, Joshua L.; Brinkley, Kelly; Sickler, Todd; Coleman, Mark; Williamson, Chatt; Gurton, Kris; Felton, Melvin; Pinnick, Ronald G.; Baker, Neal; Eshbaugh, Jonathan; Hahn, Jerry; Smith, Emily; Alvarez, Ben; Prugh, Amber; Gardner, Warren.

In: Optics Express, Vol. 22, No. 7, 2014, p. 8165-8189.

Research output: Contribution to journal › Article › peer-review

Pan, YL, Hill, SC, Santarpia, JL, Brinkley, K, Sickler, T, Coleman, M, Williamson, C, Gurton, K, Felton, M, Pinnick, RG, Baker, N, Eshbaugh, J, Hahn, J, Smith, E, Alvarez, B, Prugh, A & Gardner, W 2014, 'Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory', Optics Express, vol. 22, no. 7, pp. 8165-8189. https://doi.org/10.1364/OE.22.008165

Pan, Yong Le ; Hill, Steven C. ; Santarpia, Joshua L. ; Brinkley, Kelly ; Sickler, Todd ; Coleman, Mark ; Williamson, Chatt ; Gurton, Kris ; Felton, Melvin ; Pinnick, Ronald G. ; Baker, Neal ; Eshbaugh, Jonathan ; Hahn, Jerry ; Smith, Emily ; Alvarez, Ben ; Prugh, Amber ; Gardner, Warren. / Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory. In: Optics Express. 2014 ; Vol. 22, No. 7. pp. 8165-8189.

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abstract = "A system for measuring spectrally-resolved fluorescence cross sections of single bioaerosol particles has been developed and employed in a biological safety level 3 (BSL-3) facility at Edgewood Chemical and Biological Center (ECBC). It is used to aerosolize the slurry or solution of live agents and surrogates into dried micron-size particles, and to measure the fluorescence spectra and sizes of the particles one at a time. Spectrallyresolved fluorescence cross sections were measured for (1) bacterial spores: Bacillus anthracis Ames (BaA), B. atrophaeus var. globigii (BG) (formerly known as Bacillus globigii), B. thuringiensis israelensis (Bti), B. thuringiensis kurstaki (Btk), B. anthracis Sterne (BaS); (2) vegetative bacteria: Escherichia coli (E. coli), Pantoea agglomerans (Eh) (formerly known as Erwinia herbicola), Yersinia rohdei (Yr), Yersinia pestis CO92 (Yp); and (3) virus preparations: Venezuelan equine encephalitis TC83 (VEE) and the bacteriophage MS2. The excitation wavelengths were 266 nm, 273 nm, 280 nm, 365 nm and 405 nm.",

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