Spectroscopy of proteins on surfaces: implications for protein orientation and protein-protein interactions

Therese M. Cotton, Bernard Rospendowski, Vicki Schlegel, Robert A. Uphaus, Danli L. Wang, Lars H. Eng, Marion T. Stankovich

Research output: Contribution to journalConference article

3 Scopus citations

Abstract

Several approaches for stabilizing three hemeproteins (cytochrome c, myoglobin, and cytochrome c3) on a silver surface were developed. Methodology for preventing denaturation of complex biomolecules, such as surface modification by small molecules, is often required in order to obtain surface-enhanced resonance Raman scattering (SERRS) spectra that are relevant to the functional molecule. The optimal approach depends upon the stability of the protein. For example, cytochrome c is native on a number of different substrates (silver electrodes, sols and thin-films), whereas myoglobin is most stable on silver sols. Because SERRS detects changes in the interactions of the heme group with the protein, the greater stability of cytochrome c as compared to myoglobin probably reflects the fact that the heme is covalently bound in the former. Surface selection rules were used to analyze the orientation of the heme group of several proteins relative to the silver surface. A comparison of the changes in the relative intensities of bands in the SERRS spectra of cytochrome c indicates that the protein orientation is sensitive to the presence of lipids or other proteins (avidin) on the silver surface.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1403
Issue numberpt 1
StatePublished - Jan 1 1991
EventInternational Conference on Laser Applications in Life Sciences - Moscow, USSR
Duration: Aug 27 1990Aug 31 1990

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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