Sphingolipids in the root play an important role in regulating the leaf ionome in Arabidopsis thaliana

Dai Yin Chao, Kenneth Gable, Ming Chen, Ivan Baxter, Charles R. Dietrich, Edgar B. Cahoon, Mary Lou Guerinot, Brett Lahner, Shiyou Lü, Jonathan E. Markham, Joe Morrissey, Gongshe Han, Sita D. Gupta, Jeffrey M. Harmon, Jan G. Jaworski, Teresa M. Dunn, David E. Salt

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Sphingolipid synthesis is initiated by condensation of Ser with palmitoyl-CoA producing 3-ketodihydrosphinganine (3-KDS), which is reduced by a 3-KDS reductase to dihydrosphinganine. Ser palmitoyltransferase is essential for plant viability. Arabidopsis thaliana contains two genes (At3g06060/TSC10A and At5g19200/TSC10B) encoding proteins with significant similarity to the yeast 3-KDS reductase, Tsc10p. Heterologous expression in yeast of either Arabidopsis gene restored 3-KDS reductase activity to the yeast tsc10D mutant, confirming both as bona fide 3-KDS reductase genes. Consistent with sphingolipids having essential functions in plants, double mutant progeny lacking both genes were not recovered from crosses of single tsc10A and tsc10B mutants. Although the 3-KDS reductase genes are functionally redundant and ubiquitously expressed in Arabidopsis, 3-KDS reductase activity was reduced to 10% of wild-type levels in the loss-of-function tsc10a mutant, leading to an altered sphingolipid profile. This perturbation of sphingolipid biosynthesis in the Arabidopsis tsc10a mutant leads an altered leaf ionome, including increases in Na, K, and Rb and decreases in Mg, Ca, Fe, and Mo. Reciprocal grafting revealed that these changes in the leaf ionome are driven by the root and are associated with increases in root suberin and alterations in Fe homeostasis.

Original languageEnglish (US)
Pages (from-to)1061-1081
Number of pages21
JournalPlant Cell
Volume23
Issue number3
DOIs
StatePublished - Mar 2011

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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    Chao, D. Y., Gable, K., Chen, M., Baxter, I., Dietrich, C. R., Cahoon, E. B., Guerinot, M. L., Lahner, B., Lü, S., Markham, J. E., Morrissey, J., Han, G., Gupta, S. D., Harmon, J. M., Jaworski, J. G., Dunn, T. M., & Salt, D. E. (2011). Sphingolipids in the root play an important role in regulating the leaf ionome in Arabidopsis thaliana. Plant Cell, 23(3), 1061-1081. https://doi.org/10.1105/tpc.110.079095