Sphingosine kinase 2 is a chikungunya virus host factor co-localized with the viral replication complex

St P.atrick Reid, Sarah R. Tritsch, Krishna Kota, Chih Yuan Chiang, Lian Dong, Tara Kenny, Ernest E. Brueggemann, Michael D. Ward, Lisa H. Cazares, Sina Bavari

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Chikungunya virus (CHIKV) is a re-emerging alphavirus which causes severe and prolonged arthralgic febrile illness. The recent global spread of the virus and lack of approved therapeutic options makes it imperative to gain greater insight into the molecular mechanisms underlying CHIKV pathogenesis, in particular host factors recruited by the virus. In the current study, we identify sphingosine kinase 2 (SK2) as a CHIKV host factor co-localized with the viral replication complex (VRC) during infection. SK2 was demonstrated to co-localize with viral RNA and nonstructural proteins. Targeted impairment of SK2 expression or function significantly inhibited CHIKV infection. Furthermore, affinity purification-mass spectrometry studies revealed that SK2 associates with a number of proteins involved in cellular gene expression specifically during viral infection, suggesting a role in replication. Collectively these results identify SK2 as a novel CHIKV host factor.

Original languageEnglish (US)
Pages (from-to)e61
JournalEmerging microbes & infections
Volume4
Issue number10
DOIs
StatePublished - Oct 1 2015

Keywords

  • chikungunya virus
  • nsP3
  • sphingosine kinase 2
  • viral replication complex

ASJC Scopus subject areas

  • Parasitology
  • Epidemiology
  • Microbiology
  • Immunology
  • Drug Discovery
  • Virology
  • Infectious Diseases

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  • Cite this

    Reid, S. P. A., Tritsch, S. R., Kota, K., Chiang, C. Y., Dong, L., Kenny, T., Brueggemann, E. E., Ward, M. D., Cazares, L. H., & Bavari, S. (2015). Sphingosine kinase 2 is a chikungunya virus host factor co-localized with the viral replication complex. Emerging microbes & infections, 4(10), e61. https://doi.org/10.1038/emi.2015.61