Abstract
An equation was derived to describe the unusual chromatographic situation In which part of a sample of a pure solute eluted In the column void volume while the remainder was strongly retained. It was shown that this behavior was a natural consequence of slow diffusion or slow adsorption kinetics. Kinetic parameters were measured for an affinity chromatographic system consisting of Immobilized protein A and immunoglobulin G. By use of different immobilization methods and supports, it was found that diffusion was rate-limiting in some cases while adsorption was rate-limiting in others. One Immobilization method decreased the adsorption rate constant of protein A by an order of magnitude.
Original language | English (US) |
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Pages (from-to) | 274-279 |
Number of pages | 6 |
Journal | Analytical chemistry |
Volume | 58 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1986 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry