Spontaneous and coherent anti-Stokes Raman spectroscopy of human gastrocnemius muscle biopsies in CH-stretching region for discrimination of peripheral artery disease

X. Huang, S. Irmak, Y. F. Lu, I. Pipinos, G. Casale, J. Subbiah

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Peripheral Artery Disease (PAD) is a common manifestation of atherosclerosis, characterized by lower leg ischemia and myopathy in association with leg dysfunction. In the present study, Spontaneous and coherent anti-Stokes Raman scattering (CARS) spectroscopic techniques in CH-stretching spectral region were evaluated for discriminating healthy and diseased tissues of human gastrocnemius biopsies of control and PAD patients. Since Raman signatures of the tissues in the fingerprint region are highly complex and CH containing moieties are dense, CH-stretching limited spectral range was used to classify the diseased tissues. A total of 181 Raman spectra from 9 patients and 122 CARS spectra from 12 patients were acquired. Due to the high dimensionality of the data in Raman and CARS measurements, principal component analysis (PCA) was first performed to reduce the dimensionality of the data (6 and 9 principal scores for Raman and CARS, respectively) in the CH-stretching region, followed by a discriminant function analysis (DFA) to classify the samples into different categories based on disease severity. The CH2 and CH3 vibrational signatures were observed in the Raman and CARS spectroscopy. Raman and CARS data in conjunction with PCA-DFA analysis were capable of differentiating healthy and PAD gastrocnemius with an accuracy of 85.6% and 78.7%, respectively.

Original languageEnglish (US)
Pages (from-to)2766-2777
Number of pages12
JournalBiomedical Optics Express
Volume6
Issue number8
DOIs
StatePublished - Jul 7 2015

Keywords

  • Coherent anti-Stokes Raman scattering
  • Medical optics and biotechnology
  • Spectroscopy, Raman
  • Spectroscopy; (300.6230) Spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'Spontaneous and coherent anti-Stokes Raman spectroscopy of human gastrocnemius muscle biopsies in CH-stretching region for discrimination of peripheral artery disease'. Together they form a unique fingerprint.

Cite this