Spontaneous and induced sister chromatid exchange frequencies and cell cycle progression in lymphocytes of patients with carcinoma of the uterine cervix

Thirteen healthy females and thirteen untreated patients with carcinoma of the uterine cervix were studied for spontaneous and mitomycin C (MMC)-induced rates of sister chromatid exchange (SCE) and cell cycle progression. The mean values of spontaneous as well as MMC-induced SCE rates showed no statistically significant difference between groups. For studying cell cycle progression, cells in the M1, M2, and M3 stages were scored from the same samples. The percent values of cells in these stages, identified by the nature of differential sister chromatid staining, were found to be almost identical in normal as well as MMC-treated cultures in controls and patients. It was concluded that the presence of carcinoma of the uterine cervix in human females has no bearing either on spontaneous and MMC-induced SCE rates or on cell cycle progression in PHA-stimulated cultures of peripheral blood lymphocytes.