Spontaneous T cell mediated keratoconjunctivitis in Aire-deficient mice

S. Yeh, C. S. De Paiva, C. S. Hwang, K. Trinca, A. Lingappan, J. K. Rafati, W. J. Farley, D. Q. Li, S. C. Pflugfelder

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Background/aims: Patients with autoimmune polyendocrinopathy-candiasis- ectodermal dystrophy (APECED) develop severe keratoconjunctivitis, corneal scarring and visual loss, but the precise pathogenesis is unknown. This study evaluated the ocular surface immune cell environment, conjunctival goblet cell density and response to desiccating environmental stress of the autoimmune regulatory (Aire) gene knockout murine model of APECED. Methods: Aire-deficient and wild type (WT) mice were subjected to desiccating stress from a drafty, low-humidity environment and pharmacological inhibition of tear secretion for 5 days. Immune cell populations (CD4+, CD8+, CD11b +, CD45+) and goblet cell density were measured in ocular surface tissues and meibomian glands, and compared with baseline values. Results: Greater CD4+ T cell populations were observed in the conjunctival epithelium of Aire-deficient mice (p<0.001) compared with WT. Aire-deficient mice also had greater numbers of CD4+, CD8 +, and CD11b+ cells in the peripheral cornea at baseline and following desiccating stress. The meibomian glands of Aire-deficient mice demonstrated greater CD4+, CD8+, CD45+ and CD11b+ cells at baseline (p<0.001) and following desiccating stress. Conjunctival goblet cell density was lower at baseline and following desiccating stress in Aire-deficient compared with WT mice (p<0.001). Conclusion: Aire-deficiency leads to infiltration of CD4+ and CD8+ T cells on the ocular surface and meibomian glands, which is accompanied by goblet cell loss. Desiccating stress promotes this proinflammatory milieu. Immune-mediated mechanisms play a role in the severe blepharitis and keratoconjunctivitis in the murine model of APECED.

Original languageEnglish (US)
Pages (from-to)1260-1264
Number of pages5
JournalBritish Journal of Ophthalmology
Volume93
Issue number9
DOIs
StatePublished - Sep 2009
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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