TY - JOUR
T1 - Src regulates the activity of the mammalian formin protein FHOD1
AU - Koka, Sreenivas
AU - Minick, Gerald T.
AU - Zhou, You
AU - Westendorf, Jennifer J.
AU - Boehm, Matthew B.
PY - 2005/11/4
Y1 - 2005/11/4
N2 - The mammalian formin homology domain containing protein FHOD1 influences a variety of cellular events including cell migration, cytoskeletal arrangement, signal transduction, and gene expression. In this paper, we show that Src regulates a variety of FHOD1-associated effects. FHOD1 distribution to lamellipodia was prevented by the absence of Src. However, stress fiber formation induced by a C-terminal truncated form of FHOD1 was unaffected. Gene expression from an SRE-dependent promoter and from the skeletal actin promoter was induced by two truncated forms of FHOD1 and in both instances, inhibition of Src tyrosine kinase activity abrogated induction of gene expression. Furthermore, Src activity was necessary to maintain mRNA levels of FHOD1 itself, and as such, this finding represents the first description of mechanisms involved in the regulation of formin gene expression in mammals. In summary, we have identified Src as a key regulator of FHOD1 biology.
AB - The mammalian formin homology domain containing protein FHOD1 influences a variety of cellular events including cell migration, cytoskeletal arrangement, signal transduction, and gene expression. In this paper, we show that Src regulates a variety of FHOD1-associated effects. FHOD1 distribution to lamellipodia was prevented by the absence of Src. However, stress fiber formation induced by a C-terminal truncated form of FHOD1 was unaffected. Gene expression from an SRE-dependent promoter and from the skeletal actin promoter was induced by two truncated forms of FHOD1 and in both instances, inhibition of Src tyrosine kinase activity abrogated induction of gene expression. Furthermore, Src activity was necessary to maintain mRNA levels of FHOD1 itself, and as such, this finding represents the first description of mechanisms involved in the regulation of formin gene expression in mammals. In summary, we have identified Src as a key regulator of FHOD1 biology.
KW - Cytoskeleton
KW - FHOD1
KW - Formin
KW - Gene expression
KW - Serum response element
KW - Src
UR - http://www.scopus.com/inward/record.url?scp=25644447988&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25644447988&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.08.257
DO - 10.1016/j.bbrc.2005.08.257
M3 - Article
C2 - 16169515
AN - SCOPUS:25644447988
VL - 336
SP - 1285
EP - 1291
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -