Stabilization of cell-free RNA in blood samples using a new collection device

M. Rohan Fernando, Sheila E. Norton, Kristin K. Luna, Joel M. Lechner, Jianbing Qin

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Objective: To investigate whether a new blood collection device stabilizes cell-free RNA (cfRNA) in blood post-phlebotomy when compared to collection using K3EDTA tubes. Design and methods: Blood samples were drawn from healthy donors into K3EDTA tubes and Cell-Free RNA BCTs (BCTs) and stored at room temperature (20-25°C). At specified time points (days 0-3), plasma was separated and cfRNA was extracted. Reverse transcription real-time PCR was used to quantify mRNA for c-fos, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and for 18S rRNA. Results: Blood drawn into K3EDTA tubes showed a steady increase in RNA concentration over 3days of ex vivo incubation. Blood drawn into BCTs showed no statistically significant change in RNA copy number except for GAPDH on day 3. Conclusions: The novel chemical cocktail contained in the new device allows for the stabilization of cfRNA in blood samples at room temperature, which potentially enhances the clinical utility of cfRNA.

Original languageEnglish (US)
Pages (from-to)1497-1502
Number of pages6
JournalClinical Biochemistry
Volume45
Issue number16-17
DOIs
StatePublished - Nov 1 2012
Externally publishedYes

Keywords

  • Blood preservation
  • Messenger RNA
  • RNA stability

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Fernando, M. R., Norton, S. E., Luna, K. K., Lechner, J. M., & Qin, J. (2012). Stabilization of cell-free RNA in blood samples using a new collection device. Clinical Biochemistry, 45(16-17), 1497-1502. https://doi.org/10.1016/j.clinbiochem.2012.07.090