Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry

Dominic E. Warrino, Louis J. DeGennaro, Mary Hanson, Susan Swindells, Samuel J. Pirruccello, Wayne L. Ryan

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex® BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex® BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/μl and compared with samples collected in K 3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex® BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex® BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.

Original languageEnglish (US)
Pages (from-to)107-119
Number of pages13
JournalJournal of Immunological Methods
Volume305
Issue number2
DOIs
StatePublished - Oct 30 2005

Keywords

  • Flow cytometry
  • HIV panel
  • Immune markers
  • Stabilization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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