Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry

Dominic E. Warrino; Louis J. DeGennaro; Mary Hanson; Susan Swindells; Samuel J. Pirruccello; Wayne L. Ryan

doi:10.1016/j.jim.2005.01.021

Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry

Dominic E. Warrino, Louis J. DeGennaro, Mary Hanson, Susan Swindells, Samuel J. Pirruccello, Wayne L. Ryan

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K₃EDTA and Cyto-Chex® BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex® BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/μl and compared with samples collected in K ₃EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex® BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K₃EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex® BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.

Original language	English (US)
Pages (from-to)	107-119
Number of pages	13
Journal	Journal of Immunological Methods
Volume	305
Issue number	2
DOIs	https://doi.org/10.1016/j.jim.2005.01.021
State	Published - Oct 30 2005

Keywords

Flow cytometry
HIV panel
Immune markers
Stabilization

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jim.2005.01.021

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title = "Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry",

abstract = "We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex{\textregistered} BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex{\textregistered} BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/μl and compared with samples collected in K 3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex{\textregistered} BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex{\textregistered} BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.",

keywords = "Flow cytometry, HIV panel, Immune markers, Stabilization",

author = "Warrino, {Dominic E.} and DeGennaro, {Louis J.} and Mary Hanson and Susan Swindells and Pirruccello, {Samuel J.} and Ryan, {Wayne L.}",

year = "2005",

month = oct,

day = "30",

doi = "10.1016/j.jim.2005.01.021",

language = "English (US)",

volume = "305",

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journal = "Journal of Immunological Methods",

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T1 - Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry

AU - Warrino, Dominic E.

AU - DeGennaro, Louis J.

AU - Hanson, Mary

AU - Swindells, Susan

AU - Pirruccello, Samuel J.

AU - Ryan, Wayne L.

PY - 2005/10/30

Y1 - 2005/10/30

N2 - We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex® BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex® BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/μl and compared with samples collected in K 3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex® BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex® BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.

AB - We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex® BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex® BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/μl and compared with samples collected in K 3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex® BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex® BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.

KW - Flow cytometry

KW - HIV panel

KW - Immune markers

KW - Stabilization

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Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry

Abstract

Keywords

ASJC Scopus subject areas

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