Stable DNA-protein complexes in eukaryotic chromatin

Zoya Avramova, Roumen Tsanev

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Demembranized sperm and somatic nuclei of mammalian origin were extracted with high salt/urea/2-mercaptoethanol, treated with detergents and purified in CsCl density gradients to isolate DNA. Under these conditions a protein component still remained bound to DNA. This stable DNA-protein complex could be reduced to an oligodeoxynucleotide-peptide complex by extensive sequential digestions with DNase I and Pronase E. Chemical and enzymatic treatments of this complex indicated the presence of a phosphoester bond between DNA and a hydroxyamino acid. Two-dimensional tryptic peptide mapping revealed a remarkable similarity among the covalently linked protein components in all types of chromatin studied. These maps differed from the maps of mammalian topoisomerases I and II.

Original languageEnglish (US)
Pages (from-to)437-440
Number of pages4
JournalJournal of Molecular Biology
Volume196
Issue number2
DOIs
StatePublished - Jul 20 1987

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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