Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts

C. Guda; S. B. Lee; H. Daniell

doi:10.1007/s002990050008

Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts

C. Guda, S. B. Lee, H. Daniell

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

Bioelastic protein-based polymers (PBP) have several medical (prevention of post-surgical adhesions) and non-medical (biodegradable plastic) applications. This study compares expression levels of PBP genes (synthetic) integrated into the nuclear genome or the large single-copy (LSC) or inverted repeat (IR) region of the chloroplast genome in transgenic tobacco plants. Polymer transcripts accumulated up to 100-fold higher in the IR plants than in those of nuclear transgenic plants. Integration of foreign genes into all of the chloroplast genomes (homoplasmy) and higher levels of polymer transcripts were observed only in the IR and not in LSC transgenic plants. Expression of the polymer protein was further confirmed by Western blot analysis.

Original language	English (US)
Pages (from-to)	257-262
Number of pages	6
Journal	Plant Cell Reports
Volume	19
Issue number	3
DOIs	https://doi.org/10.1007/s002990050008
State	Published - Jan 2000
Externally published	Yes

Keywords

Biopolymer
Chloroplast transformation
Molecular farming
Protein-based polymer

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

Access to Document

10.1007/s002990050008

Cite this

@article{fa32333bdec84eb18b75b5760018710e,

title = "Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts",

abstract = "Bioelastic protein-based polymers (PBP) have several medical (prevention of post-surgical adhesions) and non-medical (biodegradable plastic) applications. This study compares expression levels of PBP genes (synthetic) integrated into the nuclear genome or the large single-copy (LSC) or inverted repeat (IR) region of the chloroplast genome in transgenic tobacco plants. Polymer transcripts accumulated up to 100-fold higher in the IR plants than in those of nuclear transgenic plants. Integration of foreign genes into all of the chloroplast genomes (homoplasmy) and higher levels of polymer transcripts were observed only in the IR and not in LSC transgenic plants. Expression of the polymer protein was further confirmed by Western blot analysis.",

keywords = "Biopolymer, Chloroplast transformation, Molecular farming, Protein-based polymer",

author = "C. Guda and Lee, {S. B.} and H. Daniell",

note = "Funding Information: Acknowledgements The authors are grateful to Mr. David Schaaf (Calgene) for help in transformation experiments, Dr. D.W. Urry (Bioelastics Research) for providing polymer antibodies and pET11D-EG121 and Dr. P. Maliga (Rutgers University, N.J.) for the plasmid pZS197. This work was supported in part by the USDA-NRICGP grants 93–37311, 95–02770, 97–02770, 98–01853 and NIH grant GM 16551–01 to HD.",

year = "2000",

month = jan,

doi = "10.1007/s002990050008",

language = "English (US)",

volume = "19",

pages = "257--262",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "3",

}

TY - JOUR

T1 - Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts

AU - Guda, C.

AU - Lee, S. B.

AU - Daniell, H.

N1 - Funding Information: Acknowledgements The authors are grateful to Mr. David Schaaf (Calgene) for help in transformation experiments, Dr. D.W. Urry (Bioelastics Research) for providing polymer antibodies and pET11D-EG121 and Dr. P. Maliga (Rutgers University, N.J.) for the plasmid pZS197. This work was supported in part by the USDA-NRICGP grants 93–37311, 95–02770, 97–02770, 98–01853 and NIH grant GM 16551–01 to HD.

PY - 2000/1

Y1 - 2000/1

N2 - Bioelastic protein-based polymers (PBP) have several medical (prevention of post-surgical adhesions) and non-medical (biodegradable plastic) applications. This study compares expression levels of PBP genes (synthetic) integrated into the nuclear genome or the large single-copy (LSC) or inverted repeat (IR) region of the chloroplast genome in transgenic tobacco plants. Polymer transcripts accumulated up to 100-fold higher in the IR plants than in those of nuclear transgenic plants. Integration of foreign genes into all of the chloroplast genomes (homoplasmy) and higher levels of polymer transcripts were observed only in the IR and not in LSC transgenic plants. Expression of the polymer protein was further confirmed by Western blot analysis.

AB - Bioelastic protein-based polymers (PBP) have several medical (prevention of post-surgical adhesions) and non-medical (biodegradable plastic) applications. This study compares expression levels of PBP genes (synthetic) integrated into the nuclear genome or the large single-copy (LSC) or inverted repeat (IR) region of the chloroplast genome in transgenic tobacco plants. Polymer transcripts accumulated up to 100-fold higher in the IR plants than in those of nuclear transgenic plants. Integration of foreign genes into all of the chloroplast genomes (homoplasmy) and higher levels of polymer transcripts were observed only in the IR and not in LSC transgenic plants. Expression of the polymer protein was further confirmed by Western blot analysis.

KW - Biopolymer

KW - Chloroplast transformation

KW - Molecular farming

KW - Protein-based polymer

UR - http://www.scopus.com/inward/record.url?scp=0033984413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033984413&partnerID=8YFLogxK

U2 - 10.1007/s002990050008

DO - 10.1007/s002990050008

M3 - Article

C2 - 30754904

AN - SCOPUS:0033984413

SN - 0721-7714

VL - 19

SP - 257

EP - 262

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 3

ER -

Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this