Stable transcriptional status in the apoptotic erythroid genome

Sanggyu Lee, Junmo Hwang, Jodie Ulaszek, Yeong C. Kim, Hui Dong, Hyung Soo Kim, Ji Woong Seok, Bo Kyung Suh, So Jeong Yim, Debra Johnson, Nong Hoon Choe, Kyu Tae Chang, Zae Young Ryoo, Charles C. Tseng, Amittha Wickrema, San Ming Wang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.

Original languageEnglish (US)
Pages (from-to)556-562
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number3
DOIs
StatePublished - Aug 3 2007

Keywords

  • Apoptosis
  • Erythroid
  • Gene expression
  • Genome
  • SAGE
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Stable transcriptional status in the apoptotic erythroid genome'. Together they form a unique fingerprint.

Cite this