Stage-dependent gene expression profiles during natural killer cell development

Hyung Sik Kang, Eun Mi Kim, Sanggyu Lee, Suk Ran Yoon, Toshihiko Kawamura, Young Cheol Lee, Sangsoo Kim, Pyung Keun Myung, Ming Wang San, Inpyo Choi

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Natural killer (NK) cells develop from hematopoietic stem cells (HSCs) in the bone marrow. To understand the molecular regulation of NK cell development, serial analysis of gene expression (SAGE) was applied to HSCs, NK precursor (pNK) cells, and mature NK cells (mNK) cultured without or with OP9 stromal cells. From 170,464 total individual tags from four SAGE libraries, 35,385 unique genes were identified. A set of genes was expressed in a stage-specific manner: 15 genes in HSCs, 30 genes in pNK cells, and 27 genes in mNK cells. Among them, lipoprotein lipase induced NK cell maturation and cytotoxic activity. Identification of genome-wide profiles of gene expression in different stages of NK cell development affords us a fundamental basis for defining the molecular network during NK cell development.

Original languageEnglish (US)
Pages (from-to)551-565
Number of pages15
JournalGenomics
Volume86
Issue number5
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Keywords

  • Development
  • LPL
  • NK cells
  • SAGE

ASJC Scopus subject areas

  • Genetics

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    Kang, H. S., Kim, E. M., Lee, S., Yoon, S. R., Kawamura, T., Lee, Y. C., Kim, S., Myung, P. K., San, M. W., & Choi, I. (2005). Stage-dependent gene expression profiles during natural killer cell development. Genomics, 86(5), 551-565. https://doi.org/10.1016/j.ygeno.2005.06.010