Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence

Indranil Chatterjee, Sigrid Schmitt, Christoph F. Batzilla, Susanne Engelmann, Andreas Keller, Michael W. Ring, Ralf Kautenburger, Wilma Ziebuhr, Michael Hecker, Klaus T. Preissner, Markus Bischoff, Richard A. Proctor, Horst P. Beck, Hans Peter Lenhof, Greg A. Somerville, Mathias Herrmann

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Staphylococcus aureus Clp ATPases (molecular chaperones) alter normal physiological functions including an aconitase-mediated effect onpost-stationary growth, acetate catabolism, and entry into death phase (Chatterjee et al., J. Bacteriol. 2005, 187, 4488-4496). In the present study, the global function of ClpC in physiology, metabolism, and late-stationary phase survival was examined using DNA microarrays and 2-D PAGE followed by MALDI-TOF MS. The results suggest that ClpC is involved in regulating the expression of genes and/or proteins of gluconeogenesis, the pentose-phosphate pathway, pyruvate metabolism, the electron transport chain, nucleotide metabolism, oxidative stress, metal ion homeostasis, stringent response, and programmed cell death. Thus, one major function of ClpC is balancing late growth phase carbon metabolism. Furthermore, these changes in carbon metabolismresult in alterations of the intracellular concentration of free NADH, the amount of cell-associated iron, and fatty acid metabolism. This study provides strong evidence for ClpC as a critical factor in staphylococcal energy metabolism, stress regulation, and late-stationary phase survival; therefore, these data provide important insight into the adaptation of S. aureus toward a persister state in chronic infections.

Original languageEnglish (US)
Pages (from-to)1152-1176
Number of pages25
JournalProteomics
Volume9
Issue number5
DOIs
StatePublished - Mar 2009

Keywords

  • ClpC
  • Metabolism
  • Persistence
  • Staphylococcus aureus
  • Tricarboxylic acid cycle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence'. Together they form a unique fingerprint.

Cite this