Stiffness and hepatocytes function in vitro

Srivatsan Kidambi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Liver fibrosis is a wound-healing response to chronic liver injury such as alcoholic/nonalcoholic fatty liver disease, and viral hepatitis with no FDA-approved treatments. Liver fibrosis results in a continual accumulation of extracellular matrix (ECM) proteins and paves the way for replacement of parenchyma with non-functional scar tissue. During liver fibrosis, alterations in hepatocytes phenotype including apoptosis, oxidative stress, and loss of metabolic function have been shown to precede fibrosis and promote hepatic stellate cell activation. Specifically, hepatocyte death, as part of the original injury, triggers a cascade of events, including pathological accumulation of ECM leading to the increased tissue stiffness during liver injury. This chapter provides an overview of the interplay of hepatocytes with stiffness using in vitro models mimicking physiological and pathological matrix rigidity to provide insight into the pivotal changes in hepatocytes physiology and the extent to which it mediates the progression of liver fibrosis. Establishing the molecular aspects of hepatocytes in the light of fibrotic liver stiffness is valuable towards development of novel therapeutic and diagnostic targets of liver fibrosis.

Original languageEnglish (US)
Title of host publicationLiver Elastography
Subtitle of host publicationClinical Use and Interpretation
PublisherSpringer International Publishing
Number of pages16
ISBN (Electronic)9783030405427
ISBN (Print)9783030405410
StatePublished - Jan 1 2020


  • Bio-mimetic models
  • Collagen
  • Fibrosis
  • Hepatocytes
  • Liver stiffness
  • Matrix
  • Matrix
  • Mechanosensors
  • Mechanotransduction
  • Metabolism
  • Polydimethylsiloxane

ASJC Scopus subject areas

  • General Medicine
  • General Biochemistry, Genetics and Molecular Biology


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