Stimulation of Na+-K+-ATPase by thyrotropin in cultured thyroid follicular cells

T. A. Pressley, S. C. Higham, L. A. Joson, D. W. Mercer

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Thyroid-stimulating hormone (TSH; thyrotropin) produces a pleiotropic response in the thyroid gland, accelerating nearly every aspect of metabolic turnover within the follicular epithelia. We examined the effects of TSH on expression of Na+-K+-ATPase in FRTL-5 cells, a cell line derived from rat thyroid. TSH (10 mU/ml) produced a nearly twofold increase in abundance of the mRNA encoding the catalytic α1-subunit within 6 h of treatment. With the four mRNAs encoding the β1-subunit, TSH produced a striking increase in abundance, but this regulation was discoordinate, and some species increased more than others. Similar increases in mRNA abundance were elicited by activators of the adenosine 3',5'-cyclic monophosphate second messenger system. In contrast to the α1- and β1-mRNAs, the abundance of the mRNA encoding the β2-subunit was unchanged with TSH after 6 h, indicating that the effects of thyrotropin were not universal or indiscriminate. Thyrotropin also caused a 76% increase in Na+-K+-ATPase activity and a 46% increase in pump-mediated transport after 48 h. These studies suggest that the changes in metabolic turnover initiated by TSH during hormone synthesis include upregulation of the Na+-K+ pump.

Original languageEnglish (US)
Pages (from-to)C1252-C1258
JournalAmerican Journal of Physiology - Cell Physiology
Issue number5 37-5
StatePublished - 1995
Externally publishedYes


  • adenosine 3',5'- cyclic monophosphate
  • ion transport
  • sodium-potassium pump
  • thyroid regulation

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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