Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-α

Shu Wu, Kerry Rodabaugh, Otoniel Martinez-Maza, Joanna M. Watson, David S. Silberstein, Cinda M. Boyer, William P. Peters, J. Brice Weinberg, Jonathan S. Berek, Robert C. Bast

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Objective: We investigated whether monocyte-derived factors could stimulate the growth of ovarian cancer cells. Study Design: Human peripheral blood monocytes or human monocyte-like cell lines THP-1 and U-937 were cultured with or without macrophage colony-stimulating factor, lipopolysaccharide, or phorbol myristate acetate. Culture supernatants or recombinant cytokines were assayed for growth stimulation of ovarian cancer cell lines by tritium-thymidine incorporation and direct cell counts followed by statistical analysis with Student t test. Results: Conditioned medium from peripheral blood monocytes or from THP-1 or U-937 cells stimulated ovarian cancer cell growth. Interleukin-1α, tumor necrosis factor-α, and interleukin-6 also stimulated ovarian cancer cell growth, whereas macrophage, granulocyte, and granulocyte-macrophage colony-stimulating factor did not. Concentrations of tumor necrosis factor, interleukin-1, and interleukin-6 in conditioned medium could not account for all the growth stimulation, and activity remained after neutralization of tumor necrosis factor, interleukin-1, and interleukin-6 with antibodies. Conclusions: Interleukin-1, interleukin-6, tumor necrosis factor, and additional monocyte factor(s) could provide paracrine growth stimulation when monocytes are attracted to ovarian cancers that produce macrophage colony-stimulating factor.

Original languageEnglish (US)
Pages (from-to)997-1007
Number of pages11
JournalAmerican Journal of Obstetrics and Gynecology
Volume166
Issue number3
DOIs
StatePublished - Mar 1992

Keywords

  • Monocyte
  • growth factor
  • ovarian cancer

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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    Wu, S., Rodabaugh, K., Martinez-Maza, O., Watson, J. M., Silberstein, D. S., Boyer, C. M., Peters, W. P., Weinberg, J. B., Berek, J. S., & Bast, R. C. (1992). Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-α. American Journal of Obstetrics and Gynecology, 166(3), 997-1007. https://doi.org/10.1016/0002-9378(92)91379-O