TY - JOUR
T1 - Stimulus and recording variables and their effects on mammalian vestibular evoked potentials
AU - Jones, Sherri M.
AU - Subramanian, Geetha
AU - Avniel, Wilma
AU - Guo, Yuqing
AU - Burkard, Robert F.
AU - Jones, Timothy A.
N1 - Funding Information:
This research was supported by NIH NIDCD R01 DC04477 (S.M.J.), NIA AG09524 (R.F.B.), and NASA Space Life Sciences NAG 5 4607 (T.A.J.).
PY - 2002/7/30
Y1 - 2002/7/30
N2 - Linear vestibular evoked potentials (VsEPs) measure the collective neural activity of the gravity receptor organs in the inner ear that respond to linear acceleration transients. The present study examined the effects of electrode placement, analog filtering, stimulus polarity and stimulus rate on linear VsEP thresholds, latencies and amplitudes recorded from mice. Two electrode-recording montages were evaluated, rostral (forebrain) to 'mastoid' and caudal (cerebellum) to 'mastoid'. VsEP thresholds and peak latencies were identical between the two recording sites; however, peak amplitudes were larger for the caudal recording montage. VsEPs were also affected by filtering. Results suggest optimum high pass filter cutoff at 100-300 Hz, and low pass filter cutoff at 10000 Hz. To evaluate stimulus rate, linear jerk pulses were presented at 9.2, 16, 25, 40 and 80 Hz. At 80 Hz, mean latencies were longer (0.350-0.450 ms) and mean amplitudes reduced (0.8-1.8 μV) for all response peaks. In 50% of animals, late peaks (P3, N3) disappeared at 80 Hz. The results offer options for VsEP recording protocols.
AB - Linear vestibular evoked potentials (VsEPs) measure the collective neural activity of the gravity receptor organs in the inner ear that respond to linear acceleration transients. The present study examined the effects of electrode placement, analog filtering, stimulus polarity and stimulus rate on linear VsEP thresholds, latencies and amplitudes recorded from mice. Two electrode-recording montages were evaluated, rostral (forebrain) to 'mastoid' and caudal (cerebellum) to 'mastoid'. VsEP thresholds and peak latencies were identical between the two recording sites; however, peak amplitudes were larger for the caudal recording montage. VsEPs were also affected by filtering. Results suggest optimum high pass filter cutoff at 100-300 Hz, and low pass filter cutoff at 10000 Hz. To evaluate stimulus rate, linear jerk pulses were presented at 9.2, 16, 25, 40 and 80 Hz. At 80 Hz, mean latencies were longer (0.350-0.450 ms) and mean amplitudes reduced (0.8-1.8 μV) for all response peaks. In 50% of animals, late peaks (P3, N3) disappeared at 80 Hz. The results offer options for VsEP recording protocols.
KW - Evoked potential recording technique
KW - Gravity receptor function
KW - Saccule
KW - Utricle
KW - Vestibular compound action potential
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U2 - 10.1016/S0165-0270(02)00125-5
DO - 10.1016/S0165-0270(02)00125-5
M3 - Article
C2 - 12191754
AN - SCOPUS:0037199265
SN - 0165-0270
VL - 118
SP - 23
EP - 31
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -