Structural and functional organization of the exopolysaccharide biosynthesis genes in Rhizobium leguminosarum bv. viciae VF39

M. R. Sadykov; T. V. Ivashina; A. A. Kanapin; M. G. Shlyapnikov; V. N. Ksenzenko

Structural and functional organization of the exopolysaccharide biosynthesis genes in Rhizobium leguminosarum bv. viciae VF39

M. R. Sadykov, T. V. Ivashina, A. A. Kanapin, M. G. Shlyapnikov, V. N. Ksenzenko

Research output: Contribution to journal › Article › peer-review

Abstract

The 7181-nt XhoI-MluI fragment of Rhizobium leguminosarum bv. viciae VF39 chromosomal DNA was sequenced. Mutations in this region disturb the synthesis of an extracellular polysaccharide and nodulation in Vicia faba roots. Computer analysis revealed eight open reading frames designated pssE,D,C,F,G,H,I and orf8. All genes showed expression in a T7 promoter/RNA polymerase system. Comparison of the deduced amino acid sequences of their protein products with known protein sequences and analysis of exopolysaccharides in mutants showed that the pssE,D,C,F,G,H,I genes code for glycosyltransferases consecutively involved in the synthesis of the octasaccharide unit of the polysaccharide.

Original language	English (US)
Pages (from-to)	665-671
Number of pages	7
Journal	Molecular Biology
Volume	32
Issue number	5
State	Published - Sep 1998
Externally published	Yes

Keywords

Genes for acidic exopolysaccharide biosynthesis
Rhizobium leguminosarum
Structural-functional organization

ASJC Scopus subject areas

Biophysics
Structural Biology

Cite this

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title = "Structural and functional organization of the exopolysaccharide biosynthesis genes in Rhizobium leguminosarum bv. viciae VF39",

abstract = "The 7181-nt XhoI-MluI fragment of Rhizobium leguminosarum bv. viciae VF39 chromosomal DNA was sequenced. Mutations in this region disturb the synthesis of an extracellular polysaccharide and nodulation in Vicia faba roots. Computer analysis revealed eight open reading frames designated pssE,D,C,F,G,H,I and orf8. All genes showed expression in a T7 promoter/RNA polymerase system. Comparison of the deduced amino acid sequences of their protein products with known protein sequences and analysis of exopolysaccharides in mutants showed that the pssE,D,C,F,G,H,I genes code for glycosyltransferases consecutively involved in the synthesis of the octasaccharide unit of the polysaccharide.",

keywords = "Genes for acidic exopolysaccharide biosynthesis, Rhizobium leguminosarum, Structural-functional organization",

author = "Sadykov, {M. R.} and Ivashina, {T. V.} and Kanapin, {A. A.} and Shlyapnikov, {M. G.} and Ksenzenko, {V. N.}",

year = "1998",

month = sep,

language = "English (US)",

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pages = "665--671",

journal = "Molecular Biology",

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TY - JOUR

T1 - Structural and functional organization of the exopolysaccharide biosynthesis genes in Rhizobium leguminosarum bv. viciae VF39

AU - Sadykov, M. R.

AU - Ivashina, T. V.

AU - Kanapin, A. A.

AU - Shlyapnikov, M. G.

AU - Ksenzenko, V. N.

PY - 1998/9

Y1 - 1998/9

N2 - The 7181-nt XhoI-MluI fragment of Rhizobium leguminosarum bv. viciae VF39 chromosomal DNA was sequenced. Mutations in this region disturb the synthesis of an extracellular polysaccharide and nodulation in Vicia faba roots. Computer analysis revealed eight open reading frames designated pssE,D,C,F,G,H,I and orf8. All genes showed expression in a T7 promoter/RNA polymerase system. Comparison of the deduced amino acid sequences of their protein products with known protein sequences and analysis of exopolysaccharides in mutants showed that the pssE,D,C,F,G,H,I genes code for glycosyltransferases consecutively involved in the synthesis of the octasaccharide unit of the polysaccharide.

AB - The 7181-nt XhoI-MluI fragment of Rhizobium leguminosarum bv. viciae VF39 chromosomal DNA was sequenced. Mutations in this region disturb the synthesis of an extracellular polysaccharide and nodulation in Vicia faba roots. Computer analysis revealed eight open reading frames designated pssE,D,C,F,G,H,I and orf8. All genes showed expression in a T7 promoter/RNA polymerase system. Comparison of the deduced amino acid sequences of their protein products with known protein sequences and analysis of exopolysaccharides in mutants showed that the pssE,D,C,F,G,H,I genes code for glycosyltransferases consecutively involved in the synthesis of the octasaccharide unit of the polysaccharide.

KW - Genes for acidic exopolysaccharide biosynthesis

KW - Rhizobium leguminosarum

KW - Structural-functional organization

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VL - 32

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Structural and functional organization of the exopolysaccharide biosynthesis genes in Rhizobium leguminosarum bv. viciae VF39

Abstract

Keywords

ASJC Scopus subject areas

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