@article{fc47473d53be4a1a9ace00b9888288bb,
title = "Structural basis of Ets1 cooperative binding to palindromic sequences on stromelysin-1 promoter DNA",
abstract = "Ets1 is a member of the Ets family of transcription factors. Ets1 is autoinhibited and its activation requires heterodimerization with a partner protein or DNA-mediated homodimerization for cooperative DNA binding. In the latter case, Ets1 molecules bind to palindromic sequences in which two Ets-binding sites (EBS) are separated by four base pairs, for example in the promoters of stromelysin-1 and p53. Interestingly, counteraction of autoinhibition requires the autoinhibitory region encoded by exon VII of the gene. The structural basis for the requirement of autoinhibitory sequences for Ets1 binding to palindromic EBS still remains unresolved. Here we report the crystal structure of two Ets1 molecules bound to an EBS palindrome of the stromelysin-1 promoter DNA, providing a plausible explanation for the requirement of exon VIIencoded sequences for Ets1 cooperative DNA binding. The proposed mechanism was verified both in vitro by surface plasmon resonance and in vivo by transcription-based assays.",
keywords = "Crystal structure, Ets domain, Ets1, Protein-DNA complex, Stromelysin-1",
author = "Babayeva, {Nigar D.} and Wilder, {Phillip J.} and Masaaki Shiina and Koshiki Mino and Michelle Desler and Kazuhiro Ogata and Angie Rizzino and Tahirov, {Tahir H.}",
note = "Funding Information: We thank M. Aumercier for pGL3 WT and pGL3 M1M2 promoter/reporter gene constructs, J. Lovelace and G.E. Borgstahl for maintenance and management of the Eppley Institute{\textquoteright}s X-ray Crystallography facility and D.G. Vassylyev for the zonal scaling instruction files. Primers and oligonucleotides for crystallization were synthesized in the Eppley Institute{\textquoteright}s Molecular Biology Core facility. The Eppley Institute{\textquoteright}s core facilities are supported by the Cancer Center Support Grant P30CA036727. This work is supported by NIGMS grant R01GM082923 to T.H.T. and the Nebraska Department of Health grant 2009-32 to A.R. This work is also based upon research conducted at the Northeastern Collaborative Access Team beamlines of the Advanced Photon Source, supported by award RR-15301 from the National Center for Research Resources at the National Institutes of Health. Use of the Advanced Photon Source is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357.",
year = "2010",
month = aug,
day = "1",
doi = "10.4161/cc.9.14.12257",
language = "English (US)",
volume = "9",
pages = "3126--3134",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "15",
}