Structural basis of transcription inhibition by antibiotic streptolydigin

Dmitry Temiakov, Nikolay Zenkin, Marina N. Vassylyeva, Anna Perederina, Tahir H. Tahirov, Ekaterina Kashkina, Maria Savkina, Savva Zorov, Vadim Nikiforov, Noriyuki Igarashi, Naohiro Matsugaki, Soichi Wakatsuki, Konstantin Severinov, Dmitry G. Vassylyev

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 Å resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the available genetic data, the inhibitor binding site is located ∼20 Å away from the RNAP active site and encompasses the bridge helix and the trigger loop, two elements that are considered to be crucial for RNAP catalytic center function. Structure-based biochemical experiments revealed additional determinants of Stl binding and demonstrated that Stl does not affect NTP substrate binding, DNA translocation, and phosphodiester bond formation. The RNAP-Stl complex structure, its comparison with the closely related substrate bound eukaryotic transcription elongation complexes, and biochemical analysis suggest an inhibitory mechanism in which Stl stabilizes catalytically inactive (preinsertion) substrate bound transcription intermediate, thereby blocking structural isomerization of RNAP to an active configuration. The results provide a basis for a design of new antibiotics utilizing the Stl-like mechanism.

Original languageEnglish (US)
Pages (from-to)655-666
Number of pages12
JournalMolecular Cell
Volume19
Issue number5
DOIs
StatePublished - Sep 2 2005

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Temiakov, D., Zenkin, N., Vassylyeva, M. N., Perederina, A., Tahirov, T. H., Kashkina, E., Savkina, M., Zorov, S., Nikiforov, V., Igarashi, N., Matsugaki, N., Wakatsuki, S., Severinov, K., & Vassylyev, D. G. (2005). Structural basis of transcription inhibition by antibiotic streptolydigin. Molecular Cell, 19(5), 655-666. https://doi.org/10.1016/j.molcel.2005.07.020