Abstract
Adeno-associated virus (AAV), unique among animal viruses in its ability to integrate into a specific chromosomal location, is a promising vector for human gene therapy. AAV Replication (Rep) protein is essential for viral replication and integration, and its amino terminal domain possesses site-specific DNA binding and endonuclease activities required for replication initiation and integration. This domain displays a novel endonuclease fold and demonstrates an unexpected structural relationship to other viral origin binding proteins such as the papillomavirus E1 protein and the SV40 T antigen. The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily.
Original language | English (US) |
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Pages (from-to) | 327-337 |
Number of pages | 11 |
Journal | Molecular Cell |
Volume | 10 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2002 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology