Structure of the bovine ET(B) endothelin receptor gene

T. Mizuno, Y. Saito, M. Itakura, F. Ito, T. Ito, E. N. Moriyama, H. Hagiwara, S. Hirose

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The structure of the gene encoding the bovine type B endothelin receptor (ET(B)) has been established and compared with those of other heptahelical receptors. The gene is present as a single copy in the bovine genome, as demonstrated by Southern blot analysis, and spans at least 36 kb. The coding region is divided into 7 exons separated by 6 introns, one of which is more than 23 kb in length. The exons correspond well to the structural domains of the receptor: the first exon encodes the first and second transmembrane domains, and each of the following transmembrane domains is encoded by a separate exon. The portion of the ET(B) protein sequence encoded by exon 3 is quite different from the corresponding ET(A) sequence, suggesting that this region is responsible for the distinct ligand specificities of the two receptor subtypes. The second intron interrupts the canonical Asp-Arg-Tyr sequence, which is located at the end of the third transmembrane domain of the heptahelical receptors, as with the substance P, substance K, dopamine D2 and dopamine D3 receptor genes. To map the 5' region of the gene and determine the start of transcription, primer-extended cDNAs were cloned and sequenced: multiple start sites were deduced with no apparent TATA box in the expected upstream region. Similar results were obtained by ribonuclease protection analysis.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalBiochemical Journal
Volume287
Issue number1
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Mizuno, T., Saito, Y., Itakura, M., Ito, F., Ito, T., Moriyama, E. N., Hagiwara, H., & Hirose, S. (1992). Structure of the bovine ET(B) endothelin receptor gene. Biochemical Journal, 287(1), 305-309. https://doi.org/10.1042/bj2870305