Structure of the N-glycans from the chlorovirus NE-JV-1

Immacolata Speciale; Irina Agarkova; Garry A. Duncan; James L. Van Etten; Cristina De Castro

doi:10.1007/s10482-017-0861-3

Structure of the N-glycans from the chlorovirus NE-JV-1

Immacolata Speciale, Irina Agarkova, Garry A. Duncan, James L. Van Etten, Cristina De Castro

Plant Pathology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Results from recent studies are breaking the paradigm that all viruses depend on their host machinery to glycosylate their proteins. Chloroviruses encode several genes involved in glycan biosynthesis and some of their capsid proteins are decorated with N-linked oligosaccharides with unique features. Here we describe the elucidation of the N-glycan structure of an unusual chlorovirus, NE-JV-1, that belongs to the Pbi group. The host for NE-JV-1 is the zoochlorella Micractinium conductrix. Spectroscopic analyses established that this N-glycan consists of a core region that is conserved in all of the chloroviruses. The one difference is that the residue 3OMe-l-rhamnose is acetylated at the O-2 position in a non-stoichiometric fashion.

Original language	English (US)
Pages (from-to)	1391-1399
Number of pages	9
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	110
Issue number	11
DOIs	https://doi.org/10.1007/s10482-017-0861-3
State	Published - Nov 1 2017

Keywords

Chlorovirus
Micractinium conductrix
NMR analysis
Pbi virus
Virus NE-JV-1

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1007/s10482-017-0861-3

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title = "Structure of the N-glycans from the chlorovirus NE-JV-1",

abstract = "Results from recent studies are breaking the paradigm that all viruses depend on their host machinery to glycosylate their proteins. Chloroviruses encode several genes involved in glycan biosynthesis and some of their capsid proteins are decorated with N-linked oligosaccharides with unique features. Here we describe the elucidation of the N-glycan structure of an unusual chlorovirus, NE-JV-1, that belongs to the Pbi group. The host for NE-JV-1 is the zoochlorella Micractinium conductrix. Spectroscopic analyses established that this N-glycan consists of a core region that is conserved in all of the chloroviruses. The one difference is that the residue 3OMe-l-rhamnose is acetylated at the O-2 position in a non-stoichiometric fashion.",

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AU - Duncan, Garry A.

AU - Van Etten, James L.

AU - De Castro, Cristina

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KW - Chlorovirus

KW - Micractinium conductrix

KW - NMR analysis

KW - Pbi virus

KW - Virus NE-JV-1

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Structure of the N-glycans from the chlorovirus NE-JV-1

Abstract

Keywords

ASJC Scopus subject areas

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