Structures of human primosome elongation complexes

Qixiang He, Andrey G. Baranovskiy, Lucia M. Morstadt, Alisa E. Lisova, Nigar D. Babayeva, Benjamin L. Lusk, Ci Ji Lim, Tahir H. Tahirov

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The synthesis of RNA–DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA–DNA primers.

Original languageEnglish (US)
Pages (from-to)579-583
Number of pages5
JournalNature Structural and Molecular Biology
Volume30
Issue number5
DOIs
StatePublished - May 2023

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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