@article{bb6e495077d0495c8657d3500d950d0f,
title = "Structures of human primosome elongation complexes",
abstract = "The synthesis of RNA–DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 {\AA} human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA–DNA primers.",
author = "Qixiang He and Baranovskiy, {Andrey G.} and Morstadt, {Lucia M.} and Lisova, {Alisa E.} and Babayeva, {Nigar D.} and Lusk, {Benjamin L.} and Lim, {Ci Ji} and Tahirov, {Tahir H.}",
note = "Funding Information: We thank members of the Lim lab and Tahirov lab for their helpful discussions. We also thank M. Cox and I. Rayment for their constructive feedback. We also thank Z. Xu for her assistance in figures preparation. This research was, in part, supported by the Cryo-EM Research Center in the Department of Biochemistry at the University of Wisconsin-Madison and the National Cancer Institute{\textquoteright}s National Cryo-EM Facility at the Frederick National Laboratory for Cancer Research under contract HSSN261200800001E. This work was supported by the National Institute of General Medical Sciences grants R35GM127085 to T.H.T. and R00GM131023 to C.L. Support for this work was also provided to C.L. by the University of Wisconsin–Madison, Office of the Vice-Chancellor for Research and Graduate Education with funding from the Wisconsin Alumni Research Foundation and the Department of Biochemistry. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2023",
month = may,
doi = "10.1038/s41594-023-00971-3",
language = "English (US)",
volume = "30",
pages = "579--583",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Research",
number = "5",
}