Studies of antibody-antigen interactions by capillary electrophoresis: A review

Annette C. Moser; Sidney Trenhaile; Kati Frankenberg

doi:10.1016/j.ymeth.2018.03.006

Studies of antibody-antigen interactions by capillary electrophoresis: A review

Annette C. Moser, Sidney Trenhaile, Kati Frankenberg

Chemistry

Research output: Contribution to journal › Review article › peer-review

24 Scopus citations

Abstract

Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

Original language	English (US)
Pages (from-to)	66-75
Number of pages	10
Journal	Methods
Volume	146
DOIs	https://doi.org/10.1016/j.ymeth.2018.03.006
State	Published - Aug 15 2018

Keywords

Affinity capillary electrophoresis
Antibody-antigen interactions
Capillary electrophoresis frontal analysis
Flow through partial filling affinity capillary electrophoresis
Multiple sequential injections of equal volumes
Partial filling affinity capillary electrophoresis

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2018.03.006

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title = "Studies of antibody-antigen interactions by capillary electrophoresis: A review",

abstract = "Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.",

keywords = "Affinity capillary electrophoresis, Antibody-antigen interactions, Capillary electrophoresis frontal analysis, Flow through partial filling affinity capillary electrophoresis, Multiple sequential injections of equal volumes, Partial filling affinity capillary electrophoresis",

author = "Moser, {Annette C.} and Sidney Trenhaile and Kati Frankenberg",

note = "Funding Information: The authors would like to thank David S. Hage for his guidance and help with this review article. This work was supported in part by the University of Nebraska at Kearney{\textquoteright}s Undergraduate Research Fellowship Program. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

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doi = "10.1016/j.ymeth.2018.03.006",

language = "English (US)",

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AU - Trenhaile, Sidney

AU - Frankenberg, Kati

N1 - Funding Information: The authors would like to thank David S. Hage for his guidance and help with this review article. This work was supported in part by the University of Nebraska at Kearney’s Undergraduate Research Fellowship Program. Publisher Copyright: © 2018 Elsevier Inc.

PY - 2018/8/15

Y1 - 2018/8/15

N2 - Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

AB - Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

KW - Affinity capillary electrophoresis

KW - Antibody-antigen interactions

KW - Capillary electrophoresis frontal analysis

KW - Flow through partial filling affinity capillary electrophoresis

KW - Multiple sequential injections of equal volumes

KW - Partial filling affinity capillary electrophoresis

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VL - 146

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JO - Methods

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ER -

Studies of antibody-antigen interactions by capillary electrophoresis: A review

Abstract

Keywords

ASJC Scopus subject areas

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