Studies of antibody-antigen interactions by capillary electrophoresis: A review

Annette C. Moser, Sidney Trenhaile, Kati Frankenberg

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

Original languageEnglish (US)
Pages (from-to)66-75
Number of pages10
JournalMethods
Volume146
DOIs
StatePublished - Aug 15 2018

Keywords

  • Affinity capillary electrophoresis
  • Antibody-antigen interactions
  • Capillary electrophoresis frontal analysis
  • Flow through partial filling affinity capillary electrophoresis
  • Multiple sequential injections of equal volumes
  • Partial filling affinity capillary electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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