TY - JOUR
T1 - Studies of metabolite-protein interactions
T2 - A review
AU - Matsuda, Ryan
AU - Bi, Cong
AU - Anguizola, Jeanethe
AU - Sobansky, Matthew
AU - Rodriguez, Elliott
AU - Vargas Badilla, John
AU - Zheng, Xiwei
AU - Hage, Benjamin
AU - Hage, David S.
N1 - Funding Information:
This research was supported by the National Institutes of Health under grants R01 GM044931 and R01 DK069629 and was conducted in facilities that were renovated under NIH grant RR015468-01 . R. Matsuda was supported, in part, under a fellowship through the Molecular Mechanisms of Disease program at the University of Nebraska.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - The study of metabolomics can provide valuable information about biochemical pathways and processes at the molecular level. There have been many reports that have examined the structure, identity and concentrations of metabolites in biological systems. However, the binding of metabolites with proteins is also of growing interest. This review examines past reports that have looked at the binding of various types of metabolites with proteins. An overview of the techniques that have been used to characterize and study metabolite-protein binding is first provided. This is followed by examples of studies that have investigated the binding of hormones, fatty acids, drugs or other xenobiotics, and their metabolites with transport proteins and receptors. These examples include reports that have considered the structure of the resulting solute-protein complexes, the nature of the binding sites, the strength of these interactions, the variations in these interactions with solute structure, and the kinetics of these reactions. The possible effects of metabolic diseases on these processes, including the impact of alterations in the structure and function of proteins, are also considered.
AB - The study of metabolomics can provide valuable information about biochemical pathways and processes at the molecular level. There have been many reports that have examined the structure, identity and concentrations of metabolites in biological systems. However, the binding of metabolites with proteins is also of growing interest. This review examines past reports that have looked at the binding of various types of metabolites with proteins. An overview of the techniques that have been used to characterize and study metabolite-protein binding is first provided. This is followed by examples of studies that have investigated the binding of hormones, fatty acids, drugs or other xenobiotics, and their metabolites with transport proteins and receptors. These examples include reports that have considered the structure of the resulting solute-protein complexes, the nature of the binding sites, the strength of these interactions, the variations in these interactions with solute structure, and the kinetics of these reactions. The possible effects of metabolic diseases on these processes, including the impact of alterations in the structure and function of proteins, are also considered.
KW - Drug-protein interactions
KW - Fatty acid-protein interactions
KW - Hormone-protein interactions
KW - Metabolomics
KW - Protein modification
KW - Xenobiotic-protein interactions
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U2 - 10.1016/j.jchromb.2013.11.043
DO - 10.1016/j.jchromb.2013.11.043
M3 - Article
C2 - 24321277
AN - SCOPUS:84904686415
SN - 1570-0232
VL - 966
SP - 48
EP - 58
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -