STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

Shengjun Li, Kan Liua, Shuxin Zhang, Xiaoyan Wang, Kestrel Rogers, Guodong Ren, Chi Zhang, Bin Yu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

MicroRNAs (miRNAs) are key regulators of gene expression. They are processed from primary miRNA transcripts (pri-miRNAs), most of which are transcribed by DNA-dependent polymerase II (Pol II). miRNA levels are precisely controlled to maintain various biological processes. Here, we report that SHORT VALVE 1 (STV1), a conserved ribosomal protein, acts in miRNA biogenesis in Arabidopsis. A portion of STV1 localizes in the nucleus and binds pri-miRNAs. Using primiR172b as a reporter, we show that STV1 binds the stem-loop flanked by a short 5′ arm within pri-miRNAs. Lack of STV1 reduces the association of pri-miRNAs with their processing complex. These data suggest that STV1 promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. Furthermore, we show that STV1 indirectly involves in the occupancy of Pol II at the promoters of miRNA coding genes (MIR) and influences MIR promoter activities. Based on these results, we propose that STV1 refines the accumulation of miRNAs through its combined effects on pri-miRNA processing and transcription. This study uncovers an extraribosomal function of STV1.

Original languageEnglish (US)
Pages (from-to)1424-1429
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number6
DOIs
StatePublished - Feb 7 2017

Keywords

  • Arabidopsis
  • Mirna biogenesis
  • Pri-mirnas
  • STV1 RNA binding

ASJC Scopus subject areas

  • General

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