Subcellular fate of proteins and nucleic acids

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Improved understanding of the molecular mechanisms of diseases results in the rational development of drugs, which are designed to act at specific sites within cells. Irrespective of its size, such a drug must safely reach not only its target cell but also the appropriate location within the target cell to achieve the desired therapeutic effect. In the simplest scenario, small molecule drugs enter cells by diffusion and reaching the desired subcellular location is comparatively unproblematic. Unlike small molecule drugs, macromolecular drugs such as proteins, nucleic acids, and synthetic polymer-drug conjugates are restricted to fewer subcellular compartments due to their large size. Macromolecules cannot cross cell membranes by simple diffusion and are usually internalized by endocytosis. The limited ability of macromolecules to enter cells is widely exploited to their advantage by attaching ligands to macromolecules that can target them to specific cells; capitalizing on the unique expression of some plasma membrane receptors and antigens in certain cells or diseases. However, even after the internalization by endocytosis, the macromolecules remain separated from the intracellular space by a biological membrane. Efficient delivery of therapeutic macromolecules to designated subcellular locations therefore greatly relies upon successful passage across biological membranes. For example, delivery of macromolecules to the most important subcellular target, which is the nucleus, requires crossing two biological membranes. Improved understanding of differences in membrane function, properties, and structure among cellular organelles contributes to better control of subcellular delivery and targeting of drugs and becomes increasingly important for the development of macromolecular therapeutics.

Original languageEnglish (US)
Title of host publicationBiomaterials for Delivery and Targeting of Proteins and Nucleic Acids
PublisherCRC Press
Pages323-350
Number of pages28
ISBN (Electronic)9780203492321
ISBN (Print)9780849323348
StatePublished - Jan 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Oupický, D. (2004). Subcellular fate of proteins and nucleic acids. In Biomaterials for Delivery and Targeting of Proteins and Nucleic Acids (pp. 323-350). CRC Press.