Subcellular localization of acid hydrolases in rat lungs

B. O. De Lumen, S. Taylor, N. Urribarri, A. L. Tappel

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10 Scopus citations

Abstract

The subcellular distribution of twelve acid hydrolases was studied in rat lung homogenates. The distribution profile of all the hydrolases, except cathepsin C (EC 3.4.4.9), is similar to that of lysosomal enzymes in rat and guinea pig liver. The microsomal marker, glucose-6-phosphatase (D-glucose-6-phosphate phosphohydrolase, EC 3.1.3.9), and mitochondrial marker, the succinate oxidase system, were localized mainly in their respective fractions. The increases of specific activities of the light-mitochondrial fraction over the homogenate specific activities are similar to those obtained for rat liver and indicates the usefulness of the differential centrifugation method described for preparation of acid hydrolase-rich fractions from lung homogenates. This study also shows some qualitative similarities in the hydrolase complements of lung and liver.

Original languageEnglish (US)
Pages (from-to)597-600
Number of pages4
JournalBBA - Enzymology
Volume268
Issue number2
DOIs
StatePublished - May 12 1972
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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