19F NMR quantitation of lens aldose reductase activity using 3-deoxy-3-fluoro-D-glucose

K. Karino, P. F. Kador, B. Berkowitz, R. S. Balaban

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In the present study we have determined the kinetics of 3-deoxy-3-fluoro-D-glucose (3-FG) as a substrate for the aldose reductase reaction in vitro. In addition, we compared the 3-deoxy-3-fluoro-sorbitol (3-FS) production rates from 3-FG in the intact lens using 19F NMR with conventional aldose reductase determinations in extracts from the same lenses. The affinity of in vitro aldose reductase for 3-FG was approximately 20 times greater (9.3 mM) than that for glucose (188 mM). An excellent correlation between the rate of 3-FS production in the intact canine lens, determined with 19F NMR, and extracted aldose reductase activity was observed. The relatively high affinity of aldose reductase for 3-FG and the correlation of 3-FS production with enzyme activity in the intact lens suggests that 3-FS production from 3-FG detected by 19F NMR could provide an accurate noninvasive determination of aldose reductase activity in the eye lens.

Original languageEnglish (US)
Pages (from-to)20970-20975
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number31
StatePublished - 1991

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Karino, K., Kador, P. F., Berkowitz, B., & Balaban, R. S. (1991). 19F NMR quantitation of lens aldose reductase activity using 3-deoxy-3-fluoro-D-glucose. Journal of Biological Chemistry, 266(31), 20970-20975.