TY - JOUR
T1 - 1H, 15N, 13C, and 13CO Assignments of Human Interleukin-4 Using Three-Dimensional Double- and Triple-Resonance Heteronuclear Magnetic Resonance Spectroscopy
AU - Powers, Robert
AU - Garrett, Daniel S.
AU - Clore, G. Marius
AU - March, Carl J.
AU - Frieden, Eric A.
AU - Gronenbom, Angela M.
PY - 1992
Y1 - 1992
N2 - The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N- and 15N/13C-labeled IL-4 with an isotope incorporation of >95% for the protein expressed in yeast. Five independent sequential connectivity pathways via one-, two-, and three-bond heteronuclear J couplings are exploited to obtain unambiguous sequential assignments. Specifically, CO(i)N(i-1), NH(i-1) correlations are observed in the HNCO experiment, the CαH(i), Cα(i)-N(i+l) correlations in the HCA(CO)N experiment, the Cα(i)-N(i+1), NH(i+1) correlations in the HNCA and HN(CO)CA experiments, the Cα(i)-N(i+1), NH(i+1) correlations in the H(CA)NH and HN(CO)HB experiments, and the Cβ(i)-N(i+1), NH(i+1) correlations in the HN(CO)HB experiments. The backbone intraresidue Cα(i)-15N(i), NH(i)correlations are provided by the 15N-edited Hartmann-Hahn (HOHAHA) and H(CA)NH experiments, the Cβ(i)-15N(i), NH(i) correlations by the 15N-edited HOHAHA and HNHB experiments, the 13Cα(i)-15N(i), NH(i) correlations by the HNCA experiment, and the CαH(i)-13Cα(i)-13CO(i) correlations by the HCACO experiment. Aliphatic side-chain spin systems are assigned by 3D 1H-13C-13C-1H correlated (HCCH-COSY) and total correlated (HCCH-TOCSY) spectroscopy. Because of the high resolution afforded by these experiments, as well as the availability of multiple sequential connectivity pathways, ambiguities associated with the limited chemical shift dispersion associated with helical proteins are readily resolved. Further, in the majority of cases (88%), four or more sequential correlations are observed between successive residues. Consequently, the interpretation of these experiments readily lends itself to semiautomated analysis which significantly simplifies and speeds up the assignment process. The assignments presented in this paper provide the essential basis for studies aimed at determining the high-resolution three-dimensional structure of IL-4 in solution.
AB - The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N- and 15N/13C-labeled IL-4 with an isotope incorporation of >95% for the protein expressed in yeast. Five independent sequential connectivity pathways via one-, two-, and three-bond heteronuclear J couplings are exploited to obtain unambiguous sequential assignments. Specifically, CO(i)N(i-1), NH(i-1) correlations are observed in the HNCO experiment, the CαH(i), Cα(i)-N(i+l) correlations in the HCA(CO)N experiment, the Cα(i)-N(i+1), NH(i+1) correlations in the HNCA and HN(CO)CA experiments, the Cα(i)-N(i+1), NH(i+1) correlations in the H(CA)NH and HN(CO)HB experiments, and the Cβ(i)-N(i+1), NH(i+1) correlations in the HN(CO)HB experiments. The backbone intraresidue Cα(i)-15N(i), NH(i)correlations are provided by the 15N-edited Hartmann-Hahn (HOHAHA) and H(CA)NH experiments, the Cβ(i)-15N(i), NH(i) correlations by the 15N-edited HOHAHA and HNHB experiments, the 13Cα(i)-15N(i), NH(i) correlations by the HNCA experiment, and the CαH(i)-13Cα(i)-13CO(i) correlations by the HCACO experiment. Aliphatic side-chain spin systems are assigned by 3D 1H-13C-13C-1H correlated (HCCH-COSY) and total correlated (HCCH-TOCSY) spectroscopy. Because of the high resolution afforded by these experiments, as well as the availability of multiple sequential connectivity pathways, ambiguities associated with the limited chemical shift dispersion associated with helical proteins are readily resolved. Further, in the majority of cases (88%), four or more sequential correlations are observed between successive residues. Consequently, the interpretation of these experiments readily lends itself to semiautomated analysis which significantly simplifies and speeds up the assignment process. The assignments presented in this paper provide the essential basis for studies aimed at determining the high-resolution three-dimensional structure of IL-4 in solution.
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U2 - 10.1021/bi00132a026
DO - 10.1021/bi00132a026
M3 - Article
C2 - 1567880
AN - SCOPUS:0026611088
SN - 0006-2960
VL - 31
SP - 4334
EP - 4346
JO - Biochemistry
JF - Biochemistry
IS - 17
ER -