Objective: To determine whether an analytically superior gonadotropin assay, the monoclonal, two-site immunometric assay, also provided superior prediction of clinical outcomes when compared with conventional RIA methodology. Design: Methods comparison study. Setting: Tertiary academic center. Patients: One hundred fifty-seven consecutive IVF patients. Intervention: Comparisons of FSH and LH levels on cycle day 3 were made using paired RIA and immunometric assay procedures. The ability of day 3 LH, FSH, and their ratio in predicting IVF performance was determined using regression analyses. Results: The predictive ability of FSH as assayed by immunometric assay at least equaled that obtained by RIA for both peak E2 levels and the number of mature oocytes retrieved. Conclusion: Results from this study indicate that gonadotropin levels as measured by immunometric assay represent an effective clinical tool in predicting IVF outcomes that may prove superior to RIA.
- immunometric assay
- predictors of IVF performance
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology