@inbook{1c9c65ad8a9548549544dd00982565ec,
title = "Supported lipid bilayers for atomic force microscopy studies",
abstract = "Nanoimaging methods, atomic force microscopy (AFM) in particular, are widely used to study the interaction of biological molecules with the supported lipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enabling visualization of proteins and other molecules on its surface. Herein, we describe protocols for SLB assembly by using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) on a mica support. Our methodology enables us to assemble defect-free POPC and POPS SLBs that remain stable for at least 8 h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.",
keywords = "Amyloid aggregation, Atomic force microscope, Nanoimaging, Supported lipid bilayer, Time-lapse imaging",
author = "Zhengjian Lv and Siddhartha Banerjee and Karen Zagorski and Lyubchenko, {Yuri L.}",
note = "Funding Information: The work at University of Nebraska Medical Center was supported by grants from the National Institutes of Health (NIH) GM096039, GM118006, and NS101504 to Y.L.L. We thank Jean-Christophe Rochet (Purdue University) for providing us with the α-syn protein. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8591-3_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "129--143",
booktitle = "Methods in Molecular Biology",
}