Supported lipid bilayers for atomic force microscopy studies

Zhengjian Lv, Siddhartha Banerjee, Karen Zagorski, Yuri L. Lyubchenko

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Nanoimaging methods, atomic force microscopy (AFM) in particular, are widely used to study the interaction of biological molecules with the supported lipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enabling visualization of proteins and other molecules on its surface. Herein, we describe protocols for SLB assembly by using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) on a mica support. Our methodology enables us to assemble defect-free POPC and POPS SLBs that remain stable for at least 8 h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages129-143
Number of pages15
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1814
ISSN (Print)1064-3745

Keywords

  • Amyloid aggregation
  • Atomic force microscope
  • Nanoimaging
  • Supported lipid bilayer
  • Time-lapse imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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