Suppression of tumor-forming ability and related traits in MCF-7 human breast cancer cells by fusion with immortal mammary epithelial cells

Deborah A. Zajchowski, Vimla Band, Douglas K. Trask, David Kling, James L. Connolly, Ruth Sager

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Somatic cell hybrids between MCF-7 human breast cancer cells and normal immortalized human mammary epithelial cells have been obtained by polyethylene glycol-mediated cell fusion. The hybrid cells are suppressed in their ability to form tumors in nude mice, as well as in traits specific to the tumorigenic MCF-7 parent: growth factor independence, tumor necrosis factor sensitivity, and pS2 gene expression. In addition, they display other characteristics of the "normal" parent, including increased expression relative to the MCF-7 cells of the genes for the extracellular matrix component fibronectin, the intermediate filament keratin 5, and the angiogenesis inhibitor thrombospondin. The levels of keratins 8 and 18 also resemble those of the nontumorigenic parent. These results provide evidence for the existence of tumor suppressor gene products in immortal mammary epithelial cells. We propose a characteristic "suppressed" tumor cell phenotype, which encompasses altered cytoarchitecture, angiogenesis capabilities, and growth factor requirements.

Original languageEnglish (US)
Pages (from-to)2314-2318
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number6
DOIs
StatePublished - Mar 1990

Keywords

  • Fibronectin
  • Keratins
  • Somatic cell hybrids
  • Thrombospondin
  • Tumor suppression

ASJC Scopus subject areas

  • General

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