Abstract
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.
Original language | English (US) |
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Pages (from-to) | 3337-3341 |
Number of pages | 5 |
Journal | Applied and environmental microbiology |
Volume | 71 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2005 |
Externally published | Yes |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Food Science
- Biotechnology
- Ecology