Surface display of recombinant proteins on Bacillus thuringiensis spores

Cheng Du, Wing C. Chan, Timothy W. McKeithan, Kenneth W. Nickerson

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.

Original languageEnglish (US)
Pages (from-to)3337-3341
Number of pages5
JournalApplied and environmental microbiology
Volume71
Issue number6
DOIs
StatePublished - Jun 2005

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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