Surface functionalization of mesoporous silica nanoparticles controls loading and release behavior of mitoxantrone

Amit Wani, Elayaraja Muthuswamy, Galbokka H.Layan Savithra, Guangzhao Mao, Stephanie Brock, David Oupický

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Purpose To investigate the effect of surface functionalization of mesoporous silica nanoparticles (MSN) on crystallization, loading, release and activity of mitoxantrone (MTX). Methods Thiol-, amine-, and mixed thiol/amine-functionalized MSN were synthesized and characterized by electron microscopy, thermogravimetry, surface area analysis, elemental analysis and zeta potential. MTX loading and release kinetics were determined in phosphate and acetate buffers (pH 7.4 and 4.5). The crystalline state ofMTX inMSN was determined by differential scanning calorimetry and X-ray diffraction. Cytotoxicity and activity of MTX loaded MSN were determined by MTS assay in MDA-MB-231 cells. Results Our results demonstrate that loading of MTX depends strongly on the type of surface functional groups in MSN. Thiol-MSN showed the highest MTX loading (18 % w/w) when compared with thiol/amine-MSN (6 % w/w) and amine-MSN (1 % w/w). MTX release was strongly dependent on the pH of the release medium and the type of surface functional group. MTX was found in the amorphous form when loaded in thiolfunctionalized MSN. No significant effect of surface modification of MSN on particle toxicity was observed. MTX loaded in MSN exhibited comparable anticancer activity in vitro as free MTX. Conclusion Surface modifications of MSN have significant effect on MTX crystallization and release behavior.

Original languageEnglish (US)
Pages (from-to)2407-2418
Number of pages12
JournalPharmaceutical Research
Volume29
Issue number9
DOIs
StatePublished - Sep 1 2012

Keywords

  • Drug delivery
  • Drug release
  • Mesoporous silica
  • Mitoxantrone
  • Nanoparticles

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

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