Abstract
This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.
Original language | English (US) |
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Pages (from-to) | 321-332 |
Number of pages | 12 |
Journal | Carbohydrate Polymers |
Volume | 66 |
Issue number | 3 |
DOIs | |
State | Published - Nov 2 2006 |
Keywords
- Albumin binding
- Chemical modification
- Chitosan
ASJC Scopus subject areas
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry