Surface modification of chitosan for selective surface-protein interaction

Anuradha Subramanian, Anand V. Rau, Hemanth Kaligotla

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.

Original languageEnglish (US)
Pages (from-to)321-332
Number of pages12
JournalCarbohydrate Polymers
Issue number3
StatePublished - Nov 2 2006


  • Albumin binding
  • Chemical modification
  • Chitosan

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


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