Surface modification of chitosan for selective surface-protein interaction

Anuradha Subramanian; Anand V. Rau; Hemanth Kaligotla

doi:10.1016/j.carbpol.2006.03.022

Surface modification of chitosan for selective surface-protein interaction

Anuradha Subramanian, Anand V. Rau, Hemanth Kaligotla

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.

Original language	English (US)
Pages (from-to)	321-332
Number of pages	12
Journal	Carbohydrate Polymers
Volume	66
Issue number	3
DOIs	https://doi.org/10.1016/j.carbpol.2006.03.022
State	Published - Nov 2 2006

Keywords

Albumin binding
Chemical modification
Chitosan

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.carbpol.2006.03.022

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title = "Surface modification of chitosan for selective surface-protein interaction",

abstract = "This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.",

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T1 - Surface modification of chitosan for selective surface-protein interaction

AU - Subramanian, Anuradha

AU - Rau, Anand V.

AU - Kaligotla, Hemanth

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N2 - This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.

AB - This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.

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KW - Chemical modification

KW - Chitosan

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Surface modification of chitosan for selective surface-protein interaction

Abstract

Keywords

ASJC Scopus subject areas

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